Mouse Neurofilament (NF) ELISA Kit | MyBioSource MBS269719 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Mouse Neurofilament (NF) ELISA Kit

catalog :

MBS269719

quantity :

48-Strip-Wells

price :

290 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS269719

products type :

ELISA Kit

products full name :

Mouse Neurofilament (NF) ELISA Kit

products short name :

Neurofilament (NF)

other names :

neurofilament light polypeptide; Neurofilament light polypeptide; neurofilament light polypeptide; neurofilament subunit NF-L; neurofilament triplet L protein; neurofilament protein, light chain; neurofilament, light polypeptide 68kDa; light molecular weight neurofilament protein; protein phosphatase 1, regulatory subunit 110; neurofilament, light polypeptide; 68 kDa neurofilament protein; Neurofilament triplet L protein

products gene name :

other gene names :

NEFL; NEFL; NFL; NF-L; NF68; CMT1F; CMT2E; PPP1R110; NF68; NFL; NF-L

uniprot entry name :

NFL_HUMAN

reactivity :

Mouse

sequence length :

543

specificity :

No cross-reaction with other factors.

storage stability :

Store all reagents at 2-8 degree C.

other info1 :

Samples: Mouse serum, plasma or Cell Culture Supernatant and organizations in the natural and recombinant NF concentration. Assay Type: Sandwich. Detection Range: 20 ng/ml-0.312 ng/ml. Sensitivity: 0.06 ng/ml.

other info2 :

Intra-assay Precision: <= 8%. Inter-assay Precision: <= 12%

products description :

Principle of the assay: This experiment use double-sandwich elisa technique and the ELISA Kit provided is typical. The pre-coated antibody is Mouse NF monoclonal antibody and the detecting antibody is polyclonal antibody with biotin labeled. Samples and biotin labeling antibody are added into ELISA plate wells and washed out with PBS or TBS. Then Avidin-peroxidase conjugates are added to ELISA wells in order; Use TMB substrate for coloring after reactant thoroughly washed out by PBS or TBS. TMB turns into blue in peroxidase catalytic and finally turns into yellow under the action of acid. The color depth and the testing factors in samples are positively correlated.

ncbi gi num :

105990539

ncbi acc num :

NP_006149.2

ncbi gb acc num :

NM_006158.4

ncbi mol weight :

61,517 Da

ncbi pathways :

Activation Of NMDA Receptor Upon Glutamate Binding And Postsynaptic Events Pathway (161033); Amyotrophic Lateral Sclerosis (ALS) Pathway (920975); Amyotrophic Lateral Sclerosis (ALS) Pathway (83099); Amyotrophic Lateral Sclerosis (ALS) Pathway (511); CREB Phosphorylation Through The Activation Of CaMKII Pathway (161040); CREB Phosphorylation Through The Activation Of Ras Pathway (161036); Neuronal System Pathway (106513); Neurotransmitter Receptor Binding And Downstream Transmission In The Postsynaptic Cell Pathway (106534); Post NMDA Receptor Activation Events Pathway (161035); Ras Activation Uopn Ca2+ Infux Through NMDA Receptor Pathway (161037)

ncbi summary :

Neurofilaments are type IV intermediate filament heteropolymers composed of light, medium, and heavy chains. Neurofilaments comprise the axoskeleton and they functionally maintain the neuronal caliber. They may also play a role in intracellular transport to axons and dendrites. This gene encodes the light chain neurofilament protein. Mutations in this gene cause Charcot-Marie-Tooth disease types 1F (CMT1F) and 2E (CMT2E), disorders of the peripheral nervous system that are characterized by distinct neuropathies. A pseudogene has been identified on chromosome Y. [provided by RefSeq, Oct 2008]

uniprot summary :

NFL: one of the three (L, M, and H) intermediate filament proteins that form neurofilaments. Neurofilaments are involved in the maintenance of neuronal caliber. NF-L is the most abundant of the three neurofilament proteins. Defects cause Charcot-Marie-Tooth disease type 1F (CMT1F). Protein type: Cytoskeletal. Chromosomal Location of Human Ortholog: 8p21. Cellular Component: TSC1-TSC2 complex; growth cone; axon; cytoplasm; neurofilament; cytosol. Molecular Function: protein binding, bridging; protein C-terminus binding; protein domain specific binding; identical protein binding; protein binding; phospholipase binding; structural constituent of cytoskeleton. Biological Process: protein polymerization; response to peptide hormone stimulus; neurofilament bundle assembly; response to toxin; intermediate filament organization; microtubule cytoskeleton organization and biogenesis; retrograde axon cargo transport; axon regeneration in the peripheral nervous system; synaptic transmission; axon transport of mitochondrion; regulation of axon diameter; positive regulation of axonogenesis; negative regulation of neuron apoptosis; anterograde axon cargo transport; neuromuscular process controlling balance; locomotion; neurite morphogenesis; response to corticosterone stimulus. Disease: Charcot-marie-tooth Disease, Demyelinating, Type 1f; Charcot-marie-tooth Disease, Axonal, Type 2e

size1 :

48-Strip-Wells

price1 :

290 USD

size2 :

96-Strip-Wells

price2 :

450

more info or order :

MyBioSource product webpage