ELISA/assay

Rat Lysozyme (LZM) ELISA Kit

MBS269673

48-Strip-Wells

290 USD

ELISA Kit

Rat Lysozyme (LZM) ELISA Kit

Lysozyme (LZM)

lysozyme (EC 3.2.1.17); Lysozyme C; lysozyme C; c-type lysozyme; 1,4-beta-N-acetylmuramidase C; lysozyme; 1,4-beta-N-acetylmuramidase C

LZM

LYZ; LYZ; LZM; LZM

LYSC_HUMAN

Rat

148

No cross-reaction with other factors.

Store all reagents at 2-8 degree C.

Samples: Rat serum, plasma or Cell Culture Supernatant and organizations in the natural and recombinant LZM concentration. Assay Type: Sandwich. Detection Range: 10 ng/ml-0.156 ng/ml. Sensitivity: 0.05 ng/ml.

Intra-assay Precision: ? 8%. Inter-assay Precision: ? 12%

Principle of the assay: This experiment use double-sandwich elisa technique and the ELISA Kit provided is typical. The pre-coated antibody is Rat LZM monoclonal antibody and the detecting antibody is polyclonal antibody with biotin labeled. Samples and biotin labeling antibody are added into ELISA plate wells and washed out with PBS or TBS. Then Avidin-peroxidase conjugates are added to ELISA wells in order; Use TMB substrate for coloring after reactant thoroughly washed out by PBS or TBS. TMB turns into blue in peroxidase catalytic and finally turns into yellow under the action of acid. The color depth and the testing factors in samples are positively correlated.

307142

AAA59536.1

16,537 Da

Amyloids Pathway (366238); C-MYB Transcription Factor Network Pathway (138073); Disease Pathway (530764); Salivary Secretion Pathway (153376); Salivary Secretion Pathway (153352)

This gene encodes human lysozyme, whose natural substrate is the bacterial cell wall peptidoglycan (cleaving the beta[1-4]glycosidic linkages between N-acetylmuramic acid and N-acetylglucosamine). Lysozyme is one of the anti-microbial agents found in human milk, and is also present in spleen, lung, kidney, white blood cells, plasma, saliva, and tears. Missense mutations in LYZ have been identified in heritable renal amyloidosis. [provided by RefSeq, Jul 2008]

LYZ: Lysozymes have primarily a bacteriolytic function; those in tissues and body fluids are associated with the monocyte- macrophage system and enhance the activity of immunoagents. Defects in LYZ are a cause of amyloidosis type 8 (AMYL8); also known as systemic non-neuropathic amyloidosis or Ostertag-type amyloidosis. AMYL8 is a hereditary generalized amyloidosis due to deposition of apolipoprotein A1, fibrinogen and lysozyme amyloids. Viscera are particularly affected. There is no involvement of the nervous system. Clinical features include renal amyloidosis resulting in nephrotic syndrome, arterial hypertension, hepatosplenomegaly, cholestasis, petechial skin rash. Belongs to the glycosyl hydrolase 22 family. Protein type: Secreted; Vesicle; Endoplasmic reticulum; Hydrolase; EC 3.2.1.17; Secreted, signal peptide. Chromosomal Location of Human Ortholog: 12q15. Cellular Component: extracellular space; extracellular region. Molecular Function: identical protein binding; lysozyme activity. Biological Process: retinal homeostasis; metabolic process; cytolysis; defense response to bacterium; inflammatory response. Disease: Amyloidosis, Familial Visceral

48-Strip-Wells

290 USD

96-Strip-Wells

450