catalog number :
MBS269658
products type :
ELISA Kit
products full name :
Mouse Bone Sialoprotein (BSP) ELISA Kit
products short name :
Bone Sialoprotein (BSP)
other names :
bone sialoprotein 2; Bone sialoprotein 2; bone sialoprotein 2; BSP II; bone sialoprotein II; cell-binding sialoprotein; integrin-binding sialoprotein; Bone sialoprotein II; BSP II; Cell-binding sialoprotein; Integrin-binding sialoprotein
other gene names :
IBSP; IBSP; BSP; BNSP; SP-II; BSP-II; BNSP; BSP II
uniprot entry name :
SIAL_HUMAN
specificity :
No cross-reaction with other factors.
storage stability :
Store all reagents at 2-8 degree C.
other info1 :
Samples: Mouse serum, plasma or Cell Culture Supernatant and organizations in the natural and recombinant BSP concentration. Assay Type: Sandwich. Detection Range: 10 ng/ml-0.156 ng/ml. Sensitivity: 0.05 ng/ml.
other info2 :
Intra-assay Precision: <= 8%. Inter-assay Precision: <= 12%
products description :
Principle of the assay: This experiment use double-sandwich elisa technique and the ELISA Kit provided is typical. The pre-coated antibody is Mouse BSP monoclonal antibody and the detecting antibody is polyclonal antibody with biotin labeled. Samples and biotin labeling antibody are added into ELISA plate wells and washed out with PBS or TBS. Then Avidin-peroxidase conjugates are added to ELISA wells in order; Use TMB substrate for coloring after reactant thoroughly washed out by PBS or TBS. TMB turns into blue in peroxidase catalytic and finally turns into yellow under the action of acid. The color depth and the testing factors in samples are positively correlated.
ncbi acc num :
NP_004958.2
ncbi gb acc num :
NM_004967.3
ncbi mol weight :
35,148 Da
ncbi pathways :
ECM Proteoglycans Pathway (833812); ECM-receptor Interaction Pathway (83068); ECM-receptor Interaction Pathway (479); Extracellular Matrix Organization Pathway (576262); Focal Adhesion Pathway (198795); Focal Adhesion Pathway (83067); Focal Adhesion Pathway (478); Integrin Cell Surface Interactions Pathway (106110); Interleukin-11 Signaling Pathway (698753); Osteoblast Signaling Pathway (198842)
ncbi summary :
The protein encoded by this gene is a major structural protein of the bone matrix. It constitutes approximately 12% of the noncollagenous proteins in human bone and is synthesized by skeletal-associated cell types, including hypertrophic chondrocytes, osteoblasts, osteocytes, and osteoclasts. The only extraskeletal site of its synthesis is the trophoblast. This protein binds to calcium and hydroxyapatite via its acidic amino acid clusters, and mediates cell attachment through an RGD sequence that recognizes the vitronectin receptor. [provided by RefSeq, Jul 2008]
uniprot summary :
Sialoprotein-2: Binds tightly to hydroxyapatite. Appears to form an integral part of the mineralized matrix. Probably important to cell-matrix interaction. Promotes Arg-Gly-Asp-dependent cell attachment. Protein type: Motility/polarity/chemotaxis; Secreted, signal peptide; Secreted; Cell adhesion. Chromosomal Location of Human Ortholog: 4q21.1. Cellular Component: extracellular space; membrane; extracellular region. Biological Process: osteoblast differentiation; extracellular matrix organization and biogenesis; biomineral formation; cell adhesion