This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
MyBioSource
product type :
ELISA/assay
product name :
Human Osteopontin (OPN) ELISA Kit
catalog :
MBS269616
quantity :
48-Strip-Wells
price :
250 USD
product information
catalog number :
MBS269616
products type :
ELISA Kit
products full name :
Human Osteopontin (OPN) ELISA Kit
products short name :
[Osteopontin (OPN)]
other names :
[osteopontin; Osteopontin; osteopontin; uropontin; nephropontin; SPP1/CALPHA1 fusion; urinary stone protein; early T-lymphocyte activation 1; osteopontin/immunoglobulin alpha 1 heavy chain constant region fusion protein; secreted phosphoprotein 1 (osteopontin, bone sialoprotein I, early T-lymphocyte activation 1); secreted phosphoprotein 1; Bone sialoprotein 1; Nephropontin; Secreted phosphoprotein 1; SPP-1; Urinary stone protein; Uropontin]
products gene name :
[OPN]
other gene names :
[SPP1; SPP1; OPN; BNSP; BSPI; ETA-1; BNSP; OPN; PSEC0156; SPP-1]
uniprot entry name :
OSTP_HUMAN
reactivity :
Human
sequence length :
300
specificity :
No cross-reaction with other factors.
storage stability :
Store all reagents at 2-8 degree C.
image1 heading :
Typical Testing Data/Standard Curve
other info1 :
Samples: Human serum, plasma or cell culture supernatant and organizations in the natural and recombinant OPN concentration. Assay Type: Sandwich. Detection Range: 10 ng/ml-0.156ng/ml. Sensitivity: 0.05 ng/ml.
other info2 :
Intra-assay Precision: <= 8%. Inter-assay Precision: <= 12%
products description :
Principle of the Assay This experiment use double-sandwich elisa technique and the ELISA Kit provided is typical. The pre-coated antibody is human OPN monoclonal antibody and the detecting antibody is polyclonal antibody with biotin labeled. Samples and biotin labeling antibody are added into ELISA plate wells and washed out with PBS or TBS. Then Avidin-peroxidase conjugates are added to ELISA wells in order; Use TMB substrate for coloring after reactant thoroughly washed out by PBS or TBS. TMB turns into blue in peroxidase catalytic and finally turns into yellow under the action of acid. The color depth and the testing factors in samples are positively correlated.
ncbi gi num :
189405
ncbi acc num :
AAA59974.1
ncbi mol weight :
33,843 Da
ncbi pathways :
BDNF Signaling Pathway (712093); Direct P53 Effectors Pathway (137939); ECM-receptor Interaction Pathway (83068); ECM-receptor Interaction Pathway (479); Endochondral Ossification Pathway (198812); Extracellular Matrix Organization Pathway (576262); FGF Signaling Pathway (137989); Focal Adhesion Pathway (198795); Focal Adhesion Pathway (83067); Focal Adhesion Pathway (478)
ncbi summary :
The protein encoded by this gene is involved in the attachment of osteoclasts to the mineralized bone matrix. The encoded protein is secreted and binds hydroxyapatite with high affinity. The osteoclast vitronectin receptor is found in the cell membrane and may be involved in the binding to this protein. This protein is also a cytokine that upregulates expression of interferon-gamma and interleukin-12. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2011]
uniprot summary :
osteopontin: Binds tightly to hydroxyapatite. Appears to form an integral part of the mineralized matrix. Probably important to cell-matrix interaction. Belongs to the osteopontin family. 5 isoforms of the human protein are produced by alternative splicing. Protein type: Secreted, signal peptide; Secreted. Chromosomal Location of Human Ortholog: 4q22.1. Cellular Component: extracellular space; cell projection; perinuclear region of cytoplasm; apical part of cell; extracellular region. Molecular Function: protein binding; extracellular matrix binding; cytokine activity. Biological Process: neutrophil chemotaxis; extracellular matrix organization and biogenesis; decidualization; osteoblast differentiation; extracellular matrix disassembly; response to vitamin D; biomineral formation; response to steroid hormone stimulus; negative regulation of collateral sprouting of intact axon in response to injury; positive regulation of bone resorption; cell adhesion; inflammatory response; embryo implantation
size1 :
48-Strip-Wells
price1 :
250 USD
size2 :
96-Strip-Wells
price2 :
365
size3 :
5x96-Strip-Wells
price3 :
1480
size4 :
10x96-Strip-Wells
price4 :
2620
company information
MyBioSource
P.O. Box 153308
San Diego, CA 92195-3308
sales@mybiosource.com
https://www.mybiosource.com
1-888-627-0165
headquarters: USA
MyBioSource, LLC was orginally founded in Vancouver by three enthusiastic scientists who are passionate about providing the world with the best reagents available. Together, they form a company with a big vision known as MyBioSource. MyBioSource is now located in San Diego, California, USA.

"MyBioSource's number 1 vision is to be the world's number 1 quality reagents provider."

Our goal is to provide researchers, scientists and customers alike with a one-stop-shop for all of their reagents needs, whether it is monoclonal antibody, polyclonal antibody, recombinant protein, peptide, etc...

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