catalog number :
MBS269545
products type :
ELISA Kit
products full name :
Human Chondroitin Sulfate (CS) ELISA Kit
products short name :
[Chondroitin Sulfate (CS)]
other names :
[chondroitin sulfate N-acetylgalactosaminyltransferase 1; Chondroitin sulfate N-acetylgalactosaminyltransferase 1; chondroitin sulfate N-acetylgalactosaminyltransferase 1; beta4GalNAcT-1; chondroitin beta1,4 N-acetylgalactosaminyltransferase; chondroitin beta-1,4-N-acetylgalactosaminyltransferase 1; chondroitin sulfate N-acetylgalactosaminyltransferase 1; Chondroitin beta-1,4-N-acetylgalactosaminyltransferase 1; Beta4GalNAcT-1]
products gene name :
[CS]
other gene names :
[CSGALNACT1; CSGALNACT1; ChGn; CSGalNAcT-1; beta4GalNAcT; CHGN; GALNACT1; UNQ656/PRO1287; CsGalNAcT-1; Beta4GalNAcT-1]
uniprot entry name :
CGAT1_HUMAN
specificity :
No cross-reaction with other factors.
storage stability :
Store all reagents at 2-8 degree C.
image1 heading :
Typical Testing Data/Standard Curve (for reference only)
other info1 :
Samples: Human Serum, Plasma Or Cell Culture Supernatant And Organizations In The Natural And Recombinant Crt Concentration. Assay Type: Sandwich. Detection Range: 10 ng/ml-0.156ng/ml. Sensitivity: 0.05 ng/ml.
other info2 :
Intra-assay Precision: <= 8%. Inter-assay Precision: <= 12%
products description :
Principle of the Assay: This experiment use double-sandwich elisa technique and the ELISA Kit provided is typical. The pre-coated antibody is human CRT monoclonal antibody and the detecting antibody is polyclonal antibody with biotin labeled. Samples and biotin labeling antibody are added into ELISA plate wells and washed out with PBS or TBS. Then Avidin-peroxidase conjugates are added to ELISA wells in order; Use TMB substrate for coloring after reactant thoroughly washed out by PBS or TBS. TMB turns into blue in peroxidase catalytic and finally turns into yellow under the action of acid. The color depth and the testing factors in samples are positively correlated.
ncbi acc num :
NP_060841.5
ncbi gb acc num :
NM_018371.4
ncbi mol weight :
33,917 Da
ncbi pathways :
Chondroitin Sulfate Biosynthesis Pathway (645309); Chondroitin Sulfate/dermatan Sulfate Metabolism Pathway (645308); Disease Pathway (530764); Glycogen Storage Diseases Pathway (980468); Glycosaminoglycan Biosynthesis - Chondroitin Sulfate / Dermatan Sulfate Pathway (82982); Glycosaminoglycan Biosynthesis - Chondroitin Sulfate / Dermatan Sulfate Pathway (356); Glycosaminoglycan Biosynthesis, Chondroitin Sulfate Backbone Pathway (413364); Glycosaminoglycan Biosynthesis, Chondroitin Sulfate Backbone Pathway (468251); Glycosaminoglycan Metabolism Pathway (645297); MPS I - Hurler Syndrome Pathway (685537)
uniprot summary :
ChGn: Transfers 1,4-N-acetylgalactosamine (GalNAc) from UDP- GalNAc to the non-reducing end of glucuronic acid (GlcUA). Required for addition of the first GalNAc to the core tetrasaccharide linker and for elongation of chondroitin chains. Important role in chondroitin chain biosynthesis in cartilage. Belongs to the chondroitin N- acetylgalactosaminyltransferase family. 3 isoforms of the human protein are produced by alternative splicing. Protein type: Glycan Metabolism - chondroitin sulfate biosynthesis; EC 2.4.1.174; Transferase; Membrane protein, integral. Chromosomal Location of Human Ortholog: 8p21.3. Cellular Component: Golgi membrane; integral to Golgi membrane; intracellular. Molecular Function: glucuronosyltransferase activity; acetylgalactosaminyltransferase activity; metal ion binding; glucuronylgalactosylproteoglycan 4-beta-N-acetylgalactosaminyltransferase activity; peptidoglycan glycosyltransferase activity; glucuronosyl-N-acetylgalactosaminyl-proteoglycan 4-beta-N-acetylgalactosaminyltransferase activity. Biological Process: nervous system development; anatomical structure morphogenesis; extracellular matrix organization and biogenesis; chondroitin sulfate biosynthetic process; glycosaminoglycan metabolic process; chondroitin sulfate proteoglycan biosynthetic process; pathogenesis; cell recognition; UDP-N-acetylgalactosamine metabolic process; chondroitin sulfate metabolic process; cell proliferation; UDP-glucuronate metabolic process; heparan sulfate proteoglycan biosynthetic process, polysaccharide chain biosynthetic process; heparin biosynthetic process; proteoglycan biosynthetic process; cartilage development; carbohydrate metabolic process; chondroitin sulfate proteoglycan biosynthetic process, polysaccharide chain biosynthetic process; dermatan sulfate proteoglycan biosynthetic process; endochondral ossification
size4 :
10x96-Strip-Wells