ELISA/assay

Mouse lumican (LUM) ELISA Kit

MBS269534

48-Strip-Wells

290 USD

ELISA Kit

Mouse lumican (LUM) ELISA Kit

lumican (LUM)

lumican; Lumican; lumican; KSPG lumican; lumican proteoglycan; keratan sulfate proteoglycan lumican; lumican; Keratan sulfate proteoglycan lumican; KSPG lumican

LUM

LUM; LUM; LDC; SLRR2D; LDC; SLRR2D; KSPG lumican

LUM_HUMAN

Mouse

338

No cross-reaction with other factors.

Store all reagents at 2-8 degree C.

Samples: Mouse serum, plasma or cell culture supernatant and organizations in the natural and recombinant LUM concentration. Assay Type: Sandwich. Detection Range: 20 ng/ml-0.312 ng/ml. Sensitivity: 0.06 ng/ml.

Intra-assay Precision: <= 8%. Inter-assay Precision: <= 12%

Principle of the Assay This experiment use double-sandwich elisa technique and the ELISA Kit provided is typical. The pre-coated antibody is Mouse LUM monoclonal antibody and the detecting antibody is polyclonal antibody with biotin labeled. Samples and biotin labeling antibody are added into ELISA plate wells and washed out with PBS or TBS. Then Avidin-peroxidase conjugates are added to ELISA wells in order; Use TMB substrate for coloring after reactant thoroughly washed out by PBS or TBS. TMB turns into blue in peroxidase catalytic and finally turns into yellow under the action of acid. The color depth and the testing factors in samples are positively correlated.

4505047

NP_002336.1

NM_002345.3

38,429 Da

Disease Pathway (530764); ECM Proteoglycans Pathway (833812); Extracellular Matrix Organization Pathway (576262); Glycosaminoglycan Metabolism Pathway (645297); Keratan Sulfate Biosynthesis Pathway (645302); Keratan Sulfate Degradation Pathway (645303); Keratan Sulfate/keratin Metabolism Pathway (645301); MPS I - Hurler Syndrome Pathway (685537); MPS II - Hunter Syndrome Pathway (685538); MPS IIIA - Sanfilippo Syndrome A Pathway (685539)

This gene encodes a member of the small leucine-rich proteoglycan (SLRP) family that includes decorin, biglycan, fibromodulin, keratocan, epiphycan, and osteoglycin. In these bifunctional molecules, the protein moiety binds collagen fibrils and the highly charged hydrophilic glycosaminoglycans regulate interfibrillar spacings. Lumican is the major keratan sulfate proteoglycan of the cornea but is also distributed in interstitial collagenous matrices throughout the body. Lumican may regulate collagen fibril organization and circumferential growth, corneal transparency, and epithelial cell migration and tissue repair. [provided by RefSeq, Jul 2008]

LUM: a member of the small leucine-rich proteoglycan (SLRP) family that includes decorin, biglycan, fibromodulin, keratocan, epiphycan, and osteoglycin. In these bifunctional molecules, the protein moiety binds collagen fibrils and the highly charged hydrophilic glycosaminoglycans regulate interfibrillar spacings. Lumican is the major keratan sulfate proteoglycan of the cornea but is also distributed in interstitial collagenous matrices throughout the body. Lumican may regulate collagen fibril organization and circumferential growth, corneal transparency, and epithelial cell migration and tissue repair. [provided by RefSeq, Jul 2008]. Protein type: Secreted; Secreted, signal peptide. Chromosomal Location of Human Ortholog: 12q21.33. Cellular Component: extracellular matrix; proteinaceous extracellular matrix; extracellular space; lysosomal lumen; fibrillar collagen; Golgi lumen; extracellular region. Molecular Function: collagen binding; protein binding; extracellular matrix structural constituent. Biological Process: keratan sulfate metabolic process; extracellular matrix organization and biogenesis; collagen fibril organization; visual perception; glycosaminoglycan metabolic process; cartilage development; carbohydrate metabolic process; keratan sulfate biosynthetic process; pathogenesis; positive regulation of transcription from RNA polymerase II promoter; keratan sulfate catabolic process; response to organic cyclic substance

48-Strip-Wells

290 USD

96-Strip-Wells

450