Porcine Elastin (ELN) ELISA Kit | MyBioSource MBS269424 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Porcine Elastin (ELN) ELISA Kit

catalog :

MBS269424

quantity :

48-Strip-Wells

price :

275 USD

more info or order :

MyBioSource product webpage

image

image 1 :

product information

catalog number :

MBS269424

products type :

ELISA Kit

products full name :

Porcine Elastin (ELN) ELISA Kit

products short name :

[Elastin (ELN)]

other names :

[elastin isoform m; Elastin; elastin; tropoelastin; elastin; Tropoelastin]

products gene name :

[ELN]

other gene names :

[ELN; ELN; WS; WBS; SVAS]

uniprot entry name :

ELN_HUMAN

reactivity :

Porcine

sequence length :

786

specificity :

No cross-reaction with other factors.

storage stability :

Store all reagents at 2-8 degree C.

image1 heading :

Typical Testing Data/Standard Curve (for reference only)

other info1 :

Samples: Porcine serum, plasma or cell culture supernatant and organizations in the natural and recombinant ELN concentration. Assay Type: Sandwich. Detection Range: 20 ng/ml-0.312 ng/ml. Sensitivity: 0.06ng/ml

other info2 :

Intra-assay Precision: <= 8%. Inter-assay Precision: <= 12%

products description :

Principle of the Assay: This experiment use double-sandwich elisa technique and the ELISA Kit provided is typical. The pre-coated antibody is Porcine ELN monoclonal antibody and the detecting antibody is polyclonal antibody with biotin labeled. Samples and biotin labeling antibody are added into ELISA plate wells and washed out with PBS or TBS. Then Avidin-peroxidase conjugates are added to ELISA wells in order; Use TMB substrate for coloring after reactant thoroughly washed out by PBS or TBS. TMB turns into blue in peroxidase catalytic and finally turns into yellow under the action of acid. The color depth and the testing factors in samples are positively correlated.

ncbi gi num :

525313599

ncbi acc num :

NP_001265868.1

ncbi gb acc num :

NM_001278939.1

ncbi mol weight :

60,980 Da

ncbi pathways :

Elastic Fibre Formation Pathway (730310); Extracellular Matrix Organization Pathway (576262); Protein Digestion And Absorption Pathway (172847); Protein Digestion And Absorption Pathway (171868)

ncbi summary :

This gene encodes a protein that is one of the two components of elastic fibers. The encoded protein is rich in hydrophobic amino acids such as glycine and proline, which form mobile hydrophobic regions bounded by crosslinks between lysine residues. Deletions and mutations in this gene are associated with supravalvular aortic stenosis (SVAS) and autosomal dominant cutis laxa. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

uniprot summary :

elastin: Major structural protein of tissues such as aorta and nuchal ligament, which must expand rapidly and recover completely. Molecular determinant of the late arterial morphogenesis, stabilizing arterial structure by regulating proliferation and organization of vascular smooth muscle. Defects in ELN are the cause of cutis laxa, autosomal dominant, type 1 (ADCL1). A connective tissue disorder characterized by loose, hyperextensible skin with decreased resilience and elasticity leading to a premature aged appearance. Face, hands, feet, joints, and torso may be differentially affected. Additional variable clinical features are gastrointestinal diverticula, hernia, and genital prolapse. Rare manifestations are pulmonary artery stenosis, aortic aneurysm, bronchiectasis, and emphysema. Defects in ELN are the cause of supravalvular aortic stenosis (SVAS). SVAS is a congenital narrowing of the ascending aorta which can occur sporadically, as an autosomal dominant condition, or as one component of Williams-Beuren syndrome. ELN is located in the Williams-Beuren syndrome (WBS) critical region. WBS results from a hemizygous deletion of several genes on chromosome 7q11.23, thought to arise as a consequence of unequal crossing over between highly homologous low-copy repeat sequences flanking the deleted region. Haploinsufficiency of ELN may be the cause of certain cardiovascular and musculo-skeletal abnormalities observed in the disease. Belongs to the elastin family. 13 isoforms of the human protein are produced by alternative splicing. Protein type: Secreted; Extracellular matrix; Secreted, signal peptide. Chromosomal Location of Human Ortholog: 7q11.23. Cellular Component: proteinaceous extracellular matrix; extracellular region. Molecular Function: protein binding; extracellular matrix structural constituent. Biological Process: extracellular matrix disassembly; cell proliferation; organ morphogenesis; extracellular matrix organization and biogenesis; blood circulation; respiratory gaseous exchange. Disease: Cutis Laxa, Autosomal Dominant 1; Williams-beuren Syndrome; Supravalvular Aortic Stenosis

size1 :

48-Strip-Wells

price1 :

275 USD

size2 :

96-Strip-Wells

price2 :

435

size3 :

5x96-Strip-Wells

price3 :

1755

size4 :

10x96-Strip-Wells

price4 :

3150

more info or order :

MyBioSource product webpage