catalog number :
MBS269394
products type :
ELISA Kit
products full name :
Rat Flagellin ELISA Kit
products short name :
[Flagellin]
other names :
[flagellin; flagellin]
other gene names :
[FliC]
specificity :
No cross-reaction with other factors.
storage stability :
Store all reagents at 2-8 degree C.
image1 heading :
Typical Testing Data/Standard Curve (for reference only)
other info1 :
Samples: Rat serum, plasma or cell culture supernatant and organizations in the natural and recombinant Flagellin concentration. Assay Type: Sandwich. Detection Range: 10 ng/ml-0.156ng/ml. Sensitivity: 0.05 ng/ml
other info2 :
Intra-assay Precision: <= 8%. Inter-assay Precision: <= 12%
products description :
Principle of the Assay: This experiment use double-sandwich elisa technique and the ELISA Kit provided is typical. The pre-coated antibody is Rat Flagellin monoclonal antibody and the detecting antibody is polyclonal antibody with biotin labeled. Samples and biotin labeling antibody are added into ELISA plate wells and washed out with PBS or TBS. Then Avidin-peroxidase conjugates are added to ELISA wells in order; Use TMB substrate for coloring after reactant thoroughly washed out by PBS or TBS. TMB turns into blue in peroxidase catalytic and finally turns into yellow under the action of acid. The color depth and the testing factors in samples are positively correlated.
ncbi acc num :
NP_866107.1
ncbi gb acc num :
NC_005027.1
ncbi pathways :
Flagellar Assembly Pathway (13314); Flagellar Assembly Pathway (439); Two-component System Pathway (13312); Two-component System Pathway (437)
size4 :
10x96-Strip-Wells