ELISA/assay

Human Vitronectin (VTN) ELISA Kit

MBS269272

48-Strip-Wells

275 USD

ELISA Kit

Human Vitronectin (VTN) ELISA Kit

Vitronectin (VTN)

vitronectin; Vitronectin; vitronectin; epibolin; S-protein; somatomedin B; complement S-protein; serum spreading factor; serum-spreading factor; vitronectin; S-protein; Serum-spreading factor; V75Cleaved into the following 3 chains:Vitronectin V65 subunit; Vitronectin V10 subunit; Somatomedin-B

VTN

VTN; VTN; VN; V75; VNT; VN

VTNC_HUMAN

Human

478

No cross-reaction with other factors.

Store all reagents at 2-8 degree C.

Samples: Human serum, plasma or Cell Culture Supernatant and organizations in the natural and recombinant VTN concentration. Assay Type: Sandwich. Detection Range: 200 ng/ml-3.12 ng/ml. Sensitivity: 0.6 ng/ml.

Intra-assay Precision: <= 8%. Inter-assay Precision: <= 12%

Principle of the Assay: This experiment use double-sandwich elisa technique and the ELISA Kit provided is typical. The pre-coated antibody is human VTN monoclonal antibody and the detecting antibody is polyclonal antibody with biotin labeled. Samples and biotin labeling antibody are added into ELISA plate wells and washed out with PBS or TBS. Then Avidin-peroxidase conjugates are added to ELISA wells in order; Use TMB substrate for coloring after reactant thoroughly washed out by PBS or TBS. TMB turns into blue in peroxidase catalytic and finally turns into yellow under the action of acid. The color depth and the testing factors in samples are positively correlated.

88853069

NP_000629.3

NM_000638.3

54,306 Da

Complement Cascade Pathway (106405); ECM Proteoglycans Pathway (833812); ECM-receptor Interaction Pathway (83068); ECM-receptor Interaction Pathway (479); Elastic Fibre Formation Pathway (730310); Extracellular Matrix Organization Pathway (576262); FOXA1 Transcription Factor Network Pathway (137979); Focal Adhesion Pathway (198795); Focal Adhesion Pathway (83067); Focal Adhesion Pathway (478)

The protein encoded by this gene is a member of the pexin family. It is found in serum and tissues and promotes cell adhesion and spreading, inhibits the membrane-damaging effect of the terminal cytolytic complement pathway, and binds to several serpin serine protease inhibitors. It is a secreted protein and exists in either a single chain form or a clipped, two chain form held together by a disulfide bond. [provided by RefSeq, Jul 2008]

VTN: Vitronectin is a cell adhesion and spreading factor found in serum and tissues. Vitronectin interact with glycosaminoglycans and proteoglycans. Is recognized by certain members of the integrin family and serves as a cell-to-substrate adhesion molecule. Inhibitor of the membrane-damaging effect of the terminal cytolytic complement pathway. Protein type: Motility/polarity/chemotaxis; Secreted; Cell adhesion; Secreted, signal peptide. Chromosomal Location of Human Ortholog: 17q11. Cellular Component: extracellular matrix; proteinaceous extracellular matrix; extracellular space; extracellular region. Molecular Function: heparin binding; integrin binding; protein binding; extracellular matrix binding; scavenger receptor activity; polysaccharide binding. Biological Process: receptor-mediated endocytosis; extracellular matrix organization and biogenesis; positive regulation of protein binding; cell-matrix adhesion; negative regulation of blood coagulation; positive regulation of smooth muscle cell migration; positive regulation of receptor-mediated endocytosis; positive regulation of vascular endothelial growth factor receptor signaling pathway; positive regulation of peptidyl-tyrosine phosphorylation; regulation of complement activation; innate immune response; cell adhesion mediated by integrin; immune response; cell adhesion

48-Strip-Wells

275 USD

96-Strip-Wells

420