ELISA/assay

Rat Nuclear matrix protein 22 (NMP-22) ELISA Kit

MBS269255

48-Strip-Wells

290 USD

ELISA Kit

Rat Nuclear matrix protein 22 (NMP-22) ELISA Kit

Nuclear matrix protein 22 (NMP-22)

Nucleoprotein TPR; Nucleoprotein TPR; nucleoprotein TPR; megator; tumor potentiating region; NPC-associated intranuclear protein; translocated promoter region protein; nuclear pore complex-associated protein TPR; translocated promoter region (to activated MET oncogene); translocated promoter region, nuclear basket protein; Megator; NPC-associated intranuclear protein; Translocated promoter region protein

NMP-22

TPR; TPR

TPR_HUMAN

Rat

2363

No cross-reaction with other factors.

Store all reagents at 2-8 degree C.

Samples: Rat serum, plasma or Cell Culture Supernatant and organizations in the natural and recombinant NMP-22 concentration. Assay Type: Sandwich. Detection Range: 20 ng/ml-0.312 ng/ml. Sensitivity: 0.06 ng/ml.

Intra-assay Precision: <= 8%. Inter-assay Precision: <= 12%

Principle of the assay: This experiment use double-sandwich elisa technique and the ELISA Kit provided is typical. The pre-coated antibody is Rat NMP-22 monoclonal antibody and the detecting antibody is polyclonal antibody with biotin labeled. Samples and biotin labeling antibody are added into ELISA plate wells and washed out with PBS or TBS. Then Avidin-peroxidase conjugates are added to ELISA wells in order; Use TMB substrate for coloring after reactant thoroughly washed out by PBS or TBS. TMB turns into blue in peroxidase catalytic and finally turns into yellow under the action of acid. The color depth and the testing factors in samples are positively correlated.

215274208

P12270.3

267,293 Da

Antiviral Mechanism By IFN-stimulated Genes Pathway (530760); Cell Cycle Pathway (530733); Cell Cycle, Mitotic Pathway (105765); Cytokine Signaling In Immune System Pathway (366171); Disease Pathway (530764); Export Of Viral Ribonucleoproteins From Nucleus Pathway (106080); Gene Expression Pathway (105937); Glucose Transport Pathway (106200); Glycogen Storage Diseases Pathway (980468); HIV Infection Pathway (105983)

This gene encodes a large coiled-coil protein that forms intranuclear filaments attached to the inner surface of nuclear pore complexes (NPCs). The protein directly interacts with several components of the NPC. It is required for the nuclear export of mRNAs and some proteins. Oncogenic fusions of the 5' end of this gene with several different kinase genes occur in some neoplasias. [provided by RefSeq, Jul 2008]

TPR: a large coiled-coil nuclear pore protein that forms filamentous structures extending from nuclear pore complexes (NPCs) into the perinucleolar region. Together with Lamina-associated polypeptide 2 (LAP2) family members and Nup153, forms structural links between the nuclear lamina and the internal nuclear matrix. In interphase, localizes to the nucleoplasmic side of the nuclear pore complex (NPC) core structure, forming a fibrous structure called the nuclear basket. Essential for normal nucleocytoplasmic transport of proteins and mRNAs, plays a role in the establishment of nuclear-peripheral chromatin compartmentalization in interphase, and in the mitotic spindle checkpoint signaling during mitosis. Involved in the quality control and retention of unspliced mRNAs in the nucleus. Involved in the formation and/or maintenance of NPC-associated perinuclear heterochromatin exclusion zones (HEZs). Implicated in nuclear export of mRNAs transcribed from heat shock gene promoters; associates both with chromatin in the HSP70 promoter and with mRNAs transcribed from this promoter under stress-induced conditions. Translocations between human TPR and TrkA, MET, and RAF genes are oncogenic. Its N-terminus is involved in activation of oncogenic kinases. Protein type: Nucleoporin; Nuclear envelope; Membrane protein, peripheral; Oncoprotein. Chromosomal Location of Human Ortholog: 1q25. Cellular Component: kinetochore; nucleoplasm; nuclear membrane; extrinsic to membrane; cytoplasmic dynein complex; cytoplasm; nuclear pore; nuclear envelope; nucleus; nuclear inclusion body. Molecular Function: mRNA binding; tubulin binding; protein binding; protein homodimerization activity; transporter activity; protein anchor; heat shock protein binding; mitogen-activated protein kinase binding; chromatin binding; nucleocytoplasmic transporter activity. Biological Process: nuclear pore complex assembly; negative regulation of translational initiation; viral reproduction; RNA export from nucleus; positive regulation of heterochromatin formation; mitotic nuclear envelope disassembly; regulation of protein stability; nuclear matrix organization and biogenesis; pathogenesis; glucose transport; negative regulation of transcription from RNA polymerase II promoter; viral infectious cycle; RNA import into nucleus; regulation of protein export from nucleus; regulation of protein import into nucleus; positive regulation of protein export from nucleus; nuclear pore organization and biogenesis; protein import into nucleus; mitotic cell cycle spindle assembly checkpoint; viral transcription; protein export from nucleus; transmembrane transport; mitosis; cytokine and chemokine mediated signaling pathway; negative regulation of RNA export from nucleus; nuclear translocation of MAPK; mRNA export from nucleus during heat stress; positive regulation of protein import into nucleus; cell division; hexose transport; carbohydrate metabolic process; gene expression; mitotic cell cycle

48-Strip-Wells

290 USD

96-Strip-Wells

450