catalog number :
MBS269168
products type :
ELISA Kit
products full name :
Porcine Macrophage Inflammatory Protein 3 Alpha (MIP-3alpha) ELISA kit
products short name :
Macrophage Inflammatory Protein 3 Alpha (MIP-3alpha)
products name syn :
Porcine Macrophage Inflammatory Protein 3 Alpha (MIP-3a) ELISA kit
other names :
macrophage inflammatory protein 3 alpha; C-C motif chemokine 20; C-C motif chemokine 20; CC chemokine LARC; beta chemokine exodus-1; small-inducible cytokine A20; macrophage inflammatory protein 3 alpha; liver and activation-regulated chemokine; small inducible cytokine subfamily A (Cys-Cys), member 20; chemokine (C-C motif) ligand 20; Beta-chemokine exodus-1; CC chemokine LARC; Liver and activation-regulated chemokine; Macrophage inflammatory protein 3 alpha; MIP-3-alpha; Small-inducible cytokine A20Cleaved into the following 3 chains:CCL20(1-67); CCL20(1-64); CCL20(2-70)
products gene name :
MIP-3alpha
other gene names :
CCL20; CCL20; CKb4; LARC; ST38; MIP3A; Exodus; MIP-3a; SCYA20; MIP-3-alpha; LARC; MIP3A; SCYA20; MIP-3-alpha
uniprot entry name :
CCL20_HUMAN
specificity :
No cross-reaction with other factors.
storage stability :
Store all reagents at 2-8 degree C.
other info1 :
Samples: Porcine serum, plasma or Cell Culture Supernatant and organizations in the natural and recombinant MIP-3alpha concentration. Assay Type: Sandwich. Detection Range: 1000 pg/ml-15.6 pg/ml. Sensitivity: 5 pg/ml.
other info2 :
Intra-assay Precision: <= 8%. Inter-assay Precision: <= 12%
products description :
Principle of the assay: This experiment use double-sandwich elisa technique and the ELISA Kit provided is typical. The pre-coated antibody is Porcine MIP-3alpha monoclonal antibody and the detecting antibody is polyclonal antibody with biotin labeled. Samples and biotin labeling antibody are added into ELISA plate wells and washed out with PBS or TBS. Then Avidin-peroxidase conjugates are added to ELISA wells in order; Use TMB substrate for coloring after reactant thoroughly washed out by PBS or TBS. TMB turns into blue in peroxidase catalytic and finally turns into yellow under the action of acid. The color depth and the testing factors in samples are positively correlated.
ncbi acc num :
AAC50943.1
ncbi mol weight :
10,691 Da
ncbi pathways :
Chemokine Receptors Bind Chemokines Pathway (106359); Chemokine Signaling Pathway (99051); Chemokine Signaling Pathway (96864); Class A/1 (Rhodopsin-like Receptors) Pathway (106357); Cytokine-cytokine Receptor Interaction Pathway (83051); Cytokine-cytokine Receptor Interaction Pathway (460); EBV LMP1 Signaling Pathway (198790); G Alpha (i) Signalling Events Pathway (119550); GPCR Downstream Signaling Pathway (119548); GPCR Ligand Binding Pathway (161020)
ncbi summary :
This antimicrobial gene belongs to the subfamily of small cytokine CC genes. Cytokines are a family of secreted proteins involved in immunoregulatory and inflammatory processes. The CC cytokines are proteins characterized by two adjacent cysteines. The protein encoded by this gene displays chemotactic activity for lymphocytes and can repress proliferation of myeloid progenitors. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2014]
uniprot summary :
CCL20: Chemotactic factor that attracts lymphocytes and, slightly, neutrophils, but not monocytes. Inhibits proliferation of myeloid progenitors in colony formation assays. May be involved in formation and function of the mucosal lymphoid tissues by attracting lymphocytes and dendritic cells towards epithelial cells. C-terminal processed forms have been shown to be equally chemotactically active for leukocytes. Possesses antibacterial activity E.coli ATCC 25922 and S.aureus ATCC 29213. Belongs to the intercrine beta (chemokine CC) family. 2 isoforms of the human protein are produced by alternative splicing. Protein type: Secreted, signal peptide; Secreted; Chemokine; Motility/polarity/chemotaxis. Chromosomal Location of Human Ortholog: 2q36.3. Cellular Component: extracellular space; extracellular region. Molecular Function: protein binding; chemokine activity. Biological Process: wound healing; cell-cell signaling; defense response to bacterium; immune response; signal transduction; positive regulation of interleukin-1 alpha biosynthetic process; inflammatory response; chemotaxis; positive regulation of nitric-oxide synthase biosynthetic process