Rat Tyrosine Hydroxylase (TH) ELISA Kit | MyBioSource MBS268957 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Rat Tyrosine Hydroxylase (TH) ELISA Kit

catalog :

MBS268957

quantity :

48-Strip-Wells

price :

290 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS268957

products type :

ELISA Kit

products full name :

Rat Tyrosine Hydroxylase (TH) ELISA Kit

products short name :

Tyrosine Hydroxylase (TH)

other names :

tyrosine hydroxylase, partial; Tyrosine 3-monooxygenase; tyrosine 3-monooxygenase; dystonia 14; tyrosine 3-hydroxylase; tyrosine hydroxylase; Tyrosine 3-hydroxylase; TH

products gene name :

other gene names :

TH; TH; TYH; DYT14; DYT5b; TYH; TH

uniprot entry name :

TY3H_HUMAN

reactivity :

Rat

sequence length :

106

specificity :

No cross-reaction with other factors.

storage stability :

Store all reagents at 2-8 degree C.

other info1 :

Samples: Rat serum, plasma or Cell Culture Supernatant and organizations in the natural and recombinant TH concentration. Assay Type: Sandwich. Detection Range: 10 ng/ml-0.156 ng/ml. Sensitivity: 0.05 ng/ml.

other info2 :

Intra-assay Precision: <= 8%. Inter-assay Precision: <= 12%

products description :

Principle of the Assay: This experiment use double-sandwich elisa technique and the ELISA Kit provided is typical. The pre-coated antibody is Rat TH monoclonal antibody and the detecting antibody is polyclonal antibody with biotin labeled. Samples and biotin labeling antibody are added into ELISA plate wells and washed out with PBS or TBS. Then Avidin-peroxidase conjugates are added to ELISA wells in order; Use TMB substrate for coloring after reactant thoroughly washed out by PBS or TBS. TMB turns into blue in peroxidase catalytic and finally turns into yellow under the action of acid. The color depth and the testing factors in samples are positively correlated.

ncbi gi num :

986909

ncbi acc num :

AAA77650.1

ncbi mol weight :

44,898 Da

ncbi pathways :

ATF-2 Transcription Factor Network Pathway (138006); Alcoholism Pathway (585563); Alcoholism Pathway (587116); Alpha-synuclein Signaling Pathway (137913); Amine-derived Hormones Pathway (160983); Amphetamine Addiction Pathway (547607); Amphetamine Addiction Pathway (550546); Biogenic Amine Synthesis Pathway (198793); Catecholamine Biosynthesis Pathway (160984); Catecholamine Biosynthesis, Tyrosine = Dopamine = Noradrenaline = Adrenaline Pathway (413357)

ncbi summary :

The protein encoded by this gene is involved in the conversion of tyrosine to dopamine. It is the rate-limiting enzyme in the synthesis of catecholamines, hence plays a key role in the physiology of adrenergic neurons. Mutations in this gene have been associated with autosomal recessive Segawa syndrome. Alternatively spliced transcript variants encoding different isoforms have been noted for this gene. [provided by RefSeq, Jul 2008]

uniprot summary :

TH: an enzyme involved in the conversion of phenylalanine to dopamine. As the rate-limiting enzyme in the synthesis of catecholamines, tyrosine hydroxylase has a key role in the physiology of adrenergic neurons. Four splice variant isoforms have been described. Protein type: Amino Acid Metabolism - tyrosine; Endoplasmic reticulum; Oxidoreductase; EC 1.14.16.2; Vesicle; Mitochondrial. Chromosomal Location of Human Ortholog: 11p15.5. Cellular Component: internal side of plasma membrane; neuron projection; smooth endoplasmic reticulum; mitochondrion; dendrite; melanosome membrane; terminal button; perikaryon; cytosol; synaptic vesicle; cytoplasm; cytoplasmic vesicle; nucleus. Molecular Function: amino acid binding; protein domain specific binding; protein binding; enzyme binding; ferric iron binding; ferrous iron binding; dopamine binding; oxygen binding; tyrosine 3-monooxygenase activity. Biological Process: heart morphogenesis; heart development; response to lipopolysaccharide; dopamine biosynthetic process; phytoalexin metabolic process; norepinephrine biosynthetic process; dopamine biosynthetic process from tyrosine; catecholamine biosynthetic process; response to electrical stimulus; epinephrine biosynthetic process; response to pyrethroid; response to corticosterone stimulus; neurotransmitter biosynthetic process; response to light stimulus; anatomical structure morphogenesis; phthalate metabolic process; mating behavior; social behavior; organ morphogenesis; eye photoreceptor cell development; response to ethanol; circadian sleep/wake cycle; response to zinc ion; response to activity; cerebral cortex development; response to water deprivation; synaptic transmission, dopaminergic; response to peptide hormone stimulus; locomotory behavior; fatty acid metabolic process; response to estradiol stimulus; response to salt stress; regulation of heart contraction; sensory perception of sound; visual perception; glycoside metabolic process; response to nutrient levels; terpene metabolic process; sphingolipid metabolic process; eating behavior; response to amphetamine; multicellular organismal aging; isoquinoline alkaloid metabolic process; response to herbicide; learning; response to ether; synaptic vesicle amine transport; memory; pigmentation; response to hypoxia; embryonic camera-type eye morphogenesis. Disease: Segawa Syndrome, Autosomal Recessive

size1 :

48-Strip-Wells

price1 :

290 USD

size2 :

96-Strip-Wells

price2 :

450

more info or order :

MyBioSource product webpage