ELISA/assay

Human Cholecystokinin (CCK) ELISA Kit

MBS268925

48-Strip-Wells

250 USD

ELISA Kit

Human Cholecystokinin (CCK) ELISA Kit

[Cholecystokinin (CCK)]

[cholecystokinin; Cholecystokinin; cholecystokinin; prepro-cholecystokinin; cholecystokinin triacontatriapeptide; cholecystokinin; (1-49)-CCK58]

[CCK]

[CCK; CCK; CCK; CCK58; CCK39; CCK33; CCK25; CCK18; CCK12; CCK8; CCK7; CCK5]

CCKN_HUMAN

Human

115

No cross-reaction with other factors.

Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

Samples: Human Serum, Plasma Or Cell Culture Supernatant And Organizations In The Natural And Recombinant Cbg Concentration. Assay Type: Sandwich. Detection Range: 200 ng/ml-3.12 ng/ml. Sensitivity: 0.6 ng/ml.

Intra-assay Precision: <= 8%. Inter-assay Precision: <= 12%

Principle of the Assay: This experiment use double-sandwich elisa technique and the ELISA Kit provided is typical. The pre-coated antibody is human CBG monoclonal antibody and the detecting antibody is polyclonal antibody with biotin labeled. Samples and biotin labeling antibody are added into ELISA plate wells and washed out with PBS or TBS. Then Avidin-peroxidase conjugates are added to ELISA wells in order; Use TMB substrate for coloring after reactant thoroughly washed out by PBS or TBS. TMB turns into blue in peroxidase catalytic and finally turns into yellow under the action of acid. The color depth and the testing factors in samples are positively correlated.

179996

AAA53094.1

12,669 Da

Class A/1 (Rhodopsin-like Receptors) Pathway (106357); G Alpha (q) Signalling Events Pathway (106043); GPCR Downstream Signaling Pathway (119548); GPCR Ligand Binding Pathway (161020); Gastrin-CREB Signalling Pathway Via PKC And MAPK (645295); Peptide Ligand-binding Receptors Pathway (106358); Signal Transduction Pathway (477114); Signaling By GPCR Pathway (106356)

Cholecystokinin is a brain/gut peptide. In the gut, it induces the release of pancreatic enzymes and the contraction of the gallbladder. In the brain, its physiologic role is unclear. The cholecystokinin pro-hormone is processed by endo- and exo-proteolytic cleavages. Two transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Mar 2010]

CCK: This peptide hormone induces gall bladder contraction and the release of pancreatic enzymes in the gut. Its function in the brain is not clear. Binding to CCK-A receptors stimulates amylase release from the pancreas, binding to CCK-B receptors stimulates gastric acid secretion. Belongs to the gastrin/cholecystokinin family. Protein type: Cell development/differentiation; Secreted, signal peptide; Secreted; Hormone. Chromosomal Location of Human Ortholog: 3p22.1. Cellular Component: axon hillock; extracellular space; axon; dendrite; extracellular region; terminal button; perikaryon. Molecular Function: neuropeptide hormone activity; protein binding; hormone activity. Biological Process: caspase activation; release of cytochrome c from mitochondria; behavioral fear response; eating behavior; neuron migration; signal transduction; negative regulation of appetite; positive regulation of protein oligomerization; positive regulation of peptidyl-tyrosine phosphorylation; positive regulation of mitochondrial depolarization; axonogenesis; protein kinase C activation; positive regulation of cell proliferation; regulation of sensory perception of pain

48-Strip-Wells

250 USD

96-Strip-Wells

365

5x96-Strip-Wells

1480

10x96-Strip-Wells

2620