product summary
Loading...
company name :
MyBioSource
product type :
ELISA/assay
product name :
Chicken Testosterone ELISA Kit
catalog :
MBS268892
quantity :
48-Strip-Wells
price :
315 USD
more info or order :
MyBioSource product webpage
product information
catalog number :
MBS268892
products type :
ELISA Kit
products full name :
Chicken Testosterone ELISA Kit
products short name :
Testosterone
other names :
testosterone 17-beta-dehydrogenase 3; Testosterone 17-beta-dehydrogenase 3; testosterone 17-beta-dehydrogenase 3; 17-beta-HSD3; 17-beta-HSD 3; 17-beta-hydroxysteroid dehydrogenase type 3; testicular 17-beta-hydroxysteroid dehydrogenase; short chain dehydrogenase/reductase family 12C, member 2; hydroxysteroid (17-beta) dehydrogenase 3; 17-beta-hydroxysteroid dehydrogenase type 3; 17-beta-HSD 3; Testicular 17-beta-hydroxysteroid dehydrogenase
other gene names :
HSD17B3; HSD17B3; EDH17B3; SDR12C2; EDH17B3; 17-beta-HSD 3
uniprot entry name :
DHB3_HUMAN
reactivity :
Chicken
sequence length :
310
specificity :
No cross-reaction with other factors.
storage stability :
Store all reagents at 2-8 degree C.
other info1 :
Samples: Chicken serum, plasma or cell culture supernatant and organizations in the natural and recombinant Testosterone concentration. Assay Type: Sandwich. Detection Range: 10 ng/ml-0.156 ng/ml. Sensitivity: 0.05 ng/ml.
other info2 :
Intra-assay Precision: <= 8%. Inter-assay Precision: <= 12%
products description :
Principle of the Assay This experiment use double-sandwich elisa technique and the ELISA Kit provided is typical. The pre-coated antibody is Chicken Testosterone monoclonal antibody and the detecting antibody is polyclonal antibody with biotin labeled. Samples and biotin labeling antibody are added into ELISA plate wells and washed out with PBS or TBS. Then Avidin-peroxidase conjugates are added to ELISA wells in order; Use TMB substrate for coloring after reactant thoroughly washed out by PBS or TBS. TMB turns into blue in peroxidase catalytic and finally turns into yellow under the action of acid. The color depth and the testing factors in samples are positively correlated.
ncbi gi num :
4557649
ncbi acc num :
NP_000188.1
ncbi gb acc num :
NM_000197.1
ncbi mol weight :
34,516 Da
ncbi pathways :
Androgen Biosynthesis Pathway (106154); Fatty Acyl-CoA Biosynthesis Pathway (106115); Fatty Acid, Triacylglycerol, And Ketone Body Metabolism Pathway (160977); Metabolic Pathways (132956); Metabolism Pathway (477135); Metabolism Of Lipids And Lipoproteins Pathway (160976); Metabolism Of Steroid Hormones And Vitamin D Pathway (106150); Prostate Cancer Pathway (755440); Steroid Biosynthesis Pathway (198866); Steroid Hormone Biosynthesis Pathway (82940)
ncbi summary :
This isoform of 17 beta-hydroxysteroid dehydrogenase is expressed predominantly in the testis and catalyzes the conversion of androstenedione to testosterone. It preferentially uses NADP as cofactor. Deficiency can result in male pseudohermaphroditism with gynecomastia. [provided by RefSeq, Jul 2008]
uniprot summary :
HSD17B3: Favors the reduction of androstenedione to testosterone. Uses NADPH while the two other EDH17B enzymes use NADH. Defects in HSD17B3 are the cause of male pseudohermaphrodism with gynecomastia (MPH). These individuals have unambiguous female external genitalia at birth, but fail to menstruate at the time of expected puberty and instead virilize as evidenced by growth of the phallus. Breast development may or may not take place. Belongs to the short-chain dehydrogenases/reductases (SDR) family. 17-beta-HSD 3 subfamily. Protein type: Lipid Metabolism - androgen and estrogen; Oxidoreductase; EC 1.1.1.64. Chromosomal Location of Human Ortholog: 9q22. Cellular Component: endoplasmic reticulum membrane; intracellular membrane-bound organelle. Molecular Function: testosterone 17-beta-dehydrogenase (NADP+) activity. Biological Process: steroid metabolic process; male genitalia development; androgen biosynthetic process. Disease: 17-beta Hydroxysteroid Dehydrogenase Iii Deficiency
size1 :
48-Strip-Wells
price1 :
315 USD
size2 :
96-Strip-Wells
price2 :
490
more info or order :
MyBioSource product webpage
company information
MyBioSource
P.O. Box 153308
San Diego, CA 92195-3308
sales@mybiosource.com
https://www.mybiosource.com
1-888-627-0165
headquarters: USA
MyBioSource, LLC was orginally founded in Vancouver by three enthusiastic scientists who are passionate about providing the world with the best reagents available. Together, they form a company with a big vision known as MyBioSource. MyBioSource is now located in San Diego, California, USA.

"MyBioSource's number 1 vision is to be the world's number 1 quality reagents provider."

Our goal is to provide researchers, scientists and customers alike with a one-stop-shop for all of their reagents needs, whether it is monoclonal antibody, polyclonal antibody, recombinant protein, peptide, etc...

"MyBioSource offers the best products at unbeatable prices."

Please spend a few minutes to browse our online catalogs and see the wide range of products available. We ship our products through our shipping/distribution facility in San Diego, California, USA.

Would you like to receive email and e-newsletter from MyBioSource about new products, special offers and events? Please click here to join our Mailing List!