Human heme oxygenase 1 (HO-1) ELISA Kit | MyBioSource MBS268886 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Human heme oxygenase 1 (HO-1) ELISA Kit

catalog :

MBS268886

quantity :

48-Strip-Wells

price :

275 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS268886

products type :

ELISA Kit

products full name :

Human heme oxygenase 1 (HO-1) ELISA Kit

products short name :

heme oxygenase 1 (HO-1)

other names :

heme oxygenase 1; Heme oxygenase 1; heme oxygenase 1; heat shock protein, 32-kD; heme oxygenase (decycling) 1

products gene name :

HO-1

other gene names :

HMOX1; HMOX1; HO-1; HSP32; HMOX1D; bK286B10; HO; HO1; HO-1

uniprot entry name :

HMOX1_HUMAN

reactivity :

Human

sequence length :

288

specificity :

No cross-reaction with other factors.

storage stability :

Store all reagents at 2-8 degree C.

other info1 :

Samples: Human serum, plasma or Cell Culture Supernatant and organizations in the natural and recombinant HO-1 concentration. Assay Type: Sandwich. Detection Range: 20 ng/ml-0.312 ng/ml. Sensitivity: 0.06 ng/ml.

other info2 :

Intra-assay Precision: <= 8%. Inter-assay Precision: <= 12%

products description :

. Principle of the assay: This experiment use double-sandwich elisa technique and the ELISA Kit provided is typical. The pre-coated antibody is human HO-1 monoclonal antibody and the detecting antibody is polyclonal antibody with biotin labeled. Samples and biotin labeling antibody are added into ELISA plate wells and washed out with PBS or TBS. Then Avidin-peroxidase conjugates are added to ELISA wells in order; Use TMB substrate for coloring after reactant thoroughly washed out by PBS or TBS. TMB turns into blue in peroxidase catalytic and finally turns into yellow under the action of acid. The color depth and the testing factors in samples are positively correlated.

ncbi gi num :

4504437

ncbi acc num :

NP_002124.1

ncbi gb acc num :

NM_002133.2

ncbi mol weight :

32,819 Da

ncbi pathways :

HIF-1 Signaling Pathway (695200); HIF-1-alpha Transcription Factor Network Pathway (138045); Heme Degradation Pathway (160971); Iron Uptake And Transport Pathway (187191); Keap1-Nrf2 Pathway (198777); Metabolism Pathway (477135); Metabolism Of Porphyrins Pathway (106325); Mineral Absorption Pathway (212237); Mineral Absorption Pathway (212220); Oxidative Stress Pathway (198916)

ncbi summary :

Heme oxygenase, an essential enzyme in heme catabolism, cleaves heme to form biliverdin, which is subsequently converted to bilirubin by biliverdin reductase, and carbon monoxide, a putative neurotransmitter. Heme oxygenase activity is induced by its substrate heme and by various nonheme substances. Heme oxygenase occurs as 2 isozymes, an inducible heme oxygenase-1 and a constitutive heme oxygenase-2. HMOX1 and HMOX2 belong to the heme oxygenase family. [provided by RefSeq, Jul 2008]

uniprot summary :

HMOX1: Heme oxygenase cleaves the heme ring at the alpha methene bridge to form biliverdin. Biliverdin is subsequently converted to bilirubin by biliverdin reductase. Under physiological conditions, the activity of heme oxygenase is highest in the spleen, where senescent erythrocytes are sequestrated and destroyed. Heme oxygenase 1 activity is highly inducible by its substrate heme and by various non-heme substances such as heavy metals, bromobenzene, endotoxin, oxidizing agents and UVA. Expressed at higher levels in renal cancer tissue than in normal tissue. Belongs to the heme oxygenase family. Protein type: Cofactor and Vitamin Metabolism - porphyrin and chlorophyll; EC 1.14.99.3; Oxidoreductase. Chromosomal Location of Human Ortholog: 22q13.1. Cellular Component: extracellular space; endoplasmic reticulum membrane; membrane; perinuclear region of cytoplasm; endoplasmic reticulum; nucleolus; caveola; nucleus; cytosol. Molecular Function: signal transducer activity; protein binding; protein homodimerization activity; enzyme binding; metal ion binding; phospholipase D activity; heme binding; heme oxygenase (decyclizing) activity. Biological Process: cell death; response to nicotine; negative regulation of smooth muscle cell proliferation; cellular iron ion homeostasis; positive regulation of smooth muscle cell proliferation; negative regulation of mast cell degranulation; positive regulation of vasodilation; excretion; erythrocyte homeostasis; regulation of transcription factor activity; heme catabolic process; regulation of blood pressure; small GTPase mediated signal transduction; porphyrin metabolic process; negative regulation of mast cell cytokine production; negative regulation of neuron apoptosis; angiogenesis; regulation of transcription from RNA polymerase II promoter in response to oxidative stress; healing during inflammatory response; transmembrane transport; protein homooligomerization; positive regulation of I-kappaB kinase/NF-kappaB cascade; negative regulation of transcription factor activity; heme oxidation; cellular response to nutrient; regulation of angiogenesis; negative regulation of leukocyte migration; negative regulation of DNA binding; positive regulation of angiogenesis; iron ion homeostasis; positive regulation of chemokine biosynthetic process; DNA damage response, signal transduction resulting in induction of apoptosis; response to hydrogen peroxide; response to estrogen stimulus; endothelial cell proliferation; response to oxidative stress; smooth muscle hyperplasia. Disease: Heme Oxygenase 1 Deficiency; Pulmonary Disease, Chronic Obstructive

size1 :

48-Strip-Wells

price1 :

275 USD

size2 :

96-Strip-Wells

price2 :

420

more info or order :

MyBioSource product webpage