ELISA/assay

Human Free Fatty Acid (FFA) ELISA Kit

MBS268756

48-Strip-Wells

250 USD

ELISA Kit

Human Free Fatty Acid (FFA) ELISA Kit

[Free Fatty Acid (FFA)]

[free fatty acid receptor 2; Free fatty acid receptor 2; free fatty acid receptor 2; G protein-coupled receptor 43; G-protein coupled receptor 43; free fatty acid activated receptor 2; free fatty acid receptor 2; G-protein coupled receptor 43]

[FFA]

[FFAR2; FFAR2; FFA2R; GPR43; GPR43]

FFAR2_HUMAN

Human

330

No cross-reaction with other factors.

Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

Samples: human serum, plasma or cell culture supernatant. Detection Range: 10 mmol/L-0.156 mmol/L. Sensitivity: 0.05 mmol/L

Intra-assay Precision: ? 8%. Inter-assay Precision: ? 12%

Principle of the Assay: This experiment use double-sandwich elisa technique and the ELISA Kit provided is typical. The pre-coated antibody is human FFA monoclonal antibody and the detecting antibody is polyclonal antibody with biotin labeled. Samples and biotin labeling antibody are added into ELISA plate wells and washed out with PBS or TBS. Then Avidin-peroxidase conjugates are added to ELISA wells in order; Use TMB substrate for coloring after reactant thoroughly washed out by PBS or TBS. TMB turns into blue in peroxidase catalytic and finally turns into yellow under the action of acid. The color depth and the testing factors in samples are positively correlated.

4885333

NP_005297.1

NM_005306.2

37,144 Da

Class A/1 (Rhodopsin-like Receptors) Pathway (106357); Free Fatty Acid Receptors Pathway (161023); G Alpha (q) Signalling Events Pathway (106043); GPCR Downstream Signaling Pathway (119548); GPCR Ligand Binding Pathway (161020); GPCRs, Class A Rhodopsin-like Pathway (198886); Gastrin-CREB Signalling Pathway Via PKC And MAPK (645295); Signal Transduction Pathway (477114); Signaling By GPCR Pathway (106356); CAMP Signaling Pathway (1017634)

This gene encodes a member of the GP40 family of G protein-coupled receptors that are clustered together on chromosome 19. The encoded protein is a receptor for short chain free fatty acids and may be involved in the inflammatory response and in regulating lipid plasma levels. [provided by RefSeq, Apr 2009]

FFAR2: Receptor for short chain fatty acids through a G(i)- protein-mediated inhibition of adenylyl cyclase and elevation of intracellular calcium. The rank order of potency for agonists of this receptor is acetate= propionate = butyrate pentanoate = formate. Belongs to the G-protein coupled receptor 1 family. Protein type: Receptor, GPCR; Membrane protein, integral; GPCR, family 1; Membrane protein, multi-pass. Chromosomal Location of Human Ortholog: 19q13.1. Cellular Component: cell projection; integral to plasma membrane; plasma membrane. Molecular Function: G-protein coupled receptor activity; protein binding; lipid binding. Biological Process: fat cell differentiation; G-protein coupled receptor protein signaling pathway; sequestering of lipid; mucosal immune response; regulation of acute inflammatory response; cell surface pattern recognition receptor signaling pathway; positive regulation of cytokine production during immune response; positive regulation of acute inflammatory response to non-antigenic stimulus; glucose homeostasis; leukocyte chemotaxis during inflammatory response; positive regulation of chemokine production

48-Strip-Wells

250 USD

96-Strip-Wells

365

5x96-Strip-Wells

1480

10x96-Strip-Wells

2620