ELISA/assay

Human Antithrombin III (ATIII) ELISA Kit

MBS268748

48-Strip-Wells

275 USD

ELISA Kit

Human Antithrombin III (ATIII) ELISA Kit

Antithrombin III (ATIII)

antithrombin-III; Antithrombin-III; antithrombin-III; serine (or cysteine) proteinase inhibitor, clade C (antithrombin), member 1; serpin peptidase inhibitor, clade C (antithrombin), member 1; Serpin C1

ATIII

SERPINC1; SERPINC1; AT3; AT3D; ATIII; THPH7; AT3; PRO0309; ATIII

ANT3_HUMAN

Human

464

No cross-reaction with other factors.

Store all reagents at 2-8 degree C.

Samples: Human serum, plasma or Cell Culture Supernatant and organizations in the natural and recombinant ATIII concentration. Assay Type: Sandwich. Detection Range: 300 ng/ml-4.7 ng/ml. Sensitivity: 0.8 ng/ml.

Intra-assay Precision: <= 8%. Inter-assay: <= 12%

Principle of the assay: This experiment use double-sandwich elisa technique and the ELISA Kit provided is typical. The pre-coated antibody is human ATIII monoclonal antibody and the detecting antibody is polyclonal antibody with biotin labeled. Samples and biotin labeling antibody are added into ELISA plate wells and washed out with PBS or TBS. Then Avidin-peroxidase conjugates are added to ELISA wells in order; Use TMB substrate for coloring after reactant thoroughly washed out by PBS or TBS. TMB turns into blue in peroxidase catalytic and finally turns into yellow under the action of acid. The color depth and the testing factors in samples are positively correlated.

4502261

NP_000479.1

NM_000488.3

52,602 Da

Common Pathway (106060); Complement And Coagulation Cascades Pathway (198880); Complement And Coagulation Cascades Pathway (83073); Complement And Coagulation Cascades Pathway (484); Formation Of Fibrin Clot (Clotting Cascade) Pathway (106057); Glypican 1 Network Pathway (138010); Hemostasis Pathway (106028)

The protein encoded by this gene is a plasma protease inhibitor and a member of the serpin superfamily. This protein inhibits thrombin as well as other activated serine proteases of the coagulation system, and it regulates the blood coagulation cascade. The protein includes two functional domains: the heparin binding-domain at the N-terminus of the mature protein, and the reactive site domain at the C-terminus. The inhibitory activity is enhanced by the presence of heparin. More than 120 mutations have been identified for this gene, many of which are known to cause antithrombin-III deficiency. [provided by RefSeq, Jul 2009]

SERPINC1: Most important serine protease inhibitor in plasma that regulates the blood coagulation cascade. AT-III inhibits thrombin, matriptase-3/TMPRSS7, as well as factors IXa, Xa and XIa. Its inhibitory activity is greatly enhanced in the presence of heparin. Defects in SERPINC1 are the cause of antithrombin III deficiency (AT3D). AT3D is an important risk factor for hereditary thrombophilia, a hemostatic disorder characterized by a tendency to recurrent thrombosis. AT3D is classified into 4 types. Type I: characterized by a 50% decrease in antigenic and functional levels. Type II: has defects affecting the thrombin- binding domain. Type III: alteration of the heparin-binding domain. Plasma AT-III antigen levels are normal in type II and III. Type IV: consists of miscellaneous group of unclassifiable mutations. Belongs to the serpin family. Protein type: Secreted; Inhibitor; Secreted, signal peptide. Chromosomal Location of Human Ortholog: 1q25.1. Cellular Component: extracellular space; plasma membrane; extracellular region. Molecular Function: serine-type endopeptidase inhibitor activity; heparin binding; protein binding; protease binding. Biological Process: negative regulation of inflammatory response; blood coagulation; response to nutrient. Disease: Antithrombin Iii Deficiency

48-Strip-Wells

275 USD

96-Strip-Wells

420