Rat Neurotrophin 3 (NT3) ELISA Kit | MyBioSource MBS268663 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Rat Neurotrophin 3 (NT3) ELISA Kit

catalog :

MBS268663

quantity :

48-Strip-Wells

price :

290 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS268663

products type :

ELISA Kit

products full name :

Rat Neurotrophin 3 (NT3) ELISA Kit

products short name :

Neurotrophin 3 (NT3)

other names :

neurotrophin-3 isoform 2 preproprotein; Neurotrophin-3; neurotrophin-3; neurotrophic factor; nerve growth factor 2; neurotrophin 3; HDNF; Nerve growth factor 2; NGF-2; Neurotrophic factor

products gene name :

NT3

other gene names :

NTF3; NTF3; NT3; HDNF; NGF2; NT-3; NGF-2; NT-3; NGF-2

uniprot entry name :

NTF3_HUMAN

reactivity :

Rat

sequence length :

257

specificity :

No cross-reaction with other factors.

storage stability :

Store all reagents at 2-8 degree C.

other info1 :

Samples: Rat serum, plasma or Cell Culture Supernatant and organizations in the natural and recombinant NT3 concentration. Assay Type: Sandwich. Detection Range: 10 ng/ml-0.156 ng/ml. Sensitivity: 0.05 ng/ml.

other info2 :

Intra-assay Precision: <= 8%. Inter-assay Precision: <= 12%

products description :

Principle of the Assay: This experiment use double-sandwich elisa technique and the ELISA Kit provided is typical. The pre-coated antibody is Rat NT3 monoclonal antibody and the detecting antibody is polyclonal antibody with biotin labeled. Samples and biotin labeling antibody are added into ELISA plate wells and washed out with PBS or TBS. Then Avidin-peroxidase conjugates are added to ELISA wells in order; Use TMB substrate for coloring after reactant thoroughly washed out by PBS or TBS. TMB turns into blue in peroxidase catalytic and finally turns into yellow under the action of acid. The color depth and the testing factors in samples are positively correlated.

ncbi gi num :

4505469

ncbi acc num :

NP_002518.1

ncbi gb acc num :

NM_002527.4

ncbi mol weight :

30,800 Da

ncbi pathways :

BDNF Signaling Pathway (712093); Integrated Pancreatic Cancer Pathway (711360); MAPK Signaling Pathway (83048); MAPK Signaling Pathway (456); Neurotrophic Factor-mediated Trk Receptor Signaling Pathway (137977); Neurotrophin Signaling Pathway (101143); Neurotrophin Signaling Pathway (100064); Trk Receptor Signaling Mediated By The MAPK Pathway (138044); P75(NTR)-mediated Signaling Pathway (138019)

ncbi summary :

The protein encoded by this gene is a member of the neurotrophin family, that controls survival and differentiation of mammalian neurons. This protein is closely related to both nerve growth factor and brain-derived neurotrophic factor. It may be involved in the maintenance of the adult nervous system, and may affect development of neurons in the embryo when it is expressed in human placenta. NTF3-deficient mice generated by gene targeting display severe movement defects of the limbs. The mature peptide of this protein is identical in all mammals examined including human, pig, rat and mouse. [provided by RefSeq, Jul 2008]

uniprot summary :

NT-3: Seems to promote the survival of visceral and proprioceptive sensory neurons. Belongs to the NGF-beta family. 2 isoforms of the human protein are produced by alternative splicing. Protein type: Secreted; Cytokine; Secreted, signal peptide. Chromosomal Location of Human Ortholog: 12p13. Cellular Component: cytoplasmic membrane-bound vesicle; extracellular region. Molecular Function: neurotrophin p75 receptor binding; growth factor activity; nerve growth factor binding; receptor binding; chemoattractant activity. Biological Process: myelination; axon guidance; epidermis development; mechanoreceptor differentiation; glial cell fate determination; regulation of neuron differentiation; activation of MAPK activity; positive regulation of receptor internalization; positive regulation of glial cell differentiation; signal transduction; enteric nervous system development; induction of positive chemotaxis; positive chemotaxis; cell-cell signaling; positive regulation of cell proliferation; negative regulation of neuron apoptosis; regulation of synaptic transmission; nervous system development; negative regulation of peptidyl-tyrosine phosphorylation; activation of protein kinase B; positive regulation of peptidyl-serine phosphorylation; peripheral nervous system development; positive regulation of peptidyl-tyrosine phosphorylation; neuromuscular synaptic transmission; positive regulation of transcription from RNA polymerase II promoter; smooth muscle cell differentiation; nerve development; brain development; neurite morphogenesis; transmembrane receptor protein tyrosine kinase signaling pathway; positive regulation of cell migration

size1 :

48-Strip-Wells

price1 :

290 USD

size2 :

96-Strip-Wells

price2 :

450

more info or order :

MyBioSource product webpage