ELISA/assay

Goat Testosterone ELISA Kit

MBS268660

48-Strip-Wells

315 USD

ELISA Kit

Goat Testosterone ELISA Kit

Testosterone

testosterone 17-beta-dehydrogenase 3; Testosterone 17-beta-dehydrogenase 3; testosterone 17-beta-dehydrogenase 3; 17-beta-HSD3; 17-beta-HSD 3; 17-beta-hydroxysteroid dehydrogenase type 3; testicular 17-beta-hydroxysteroid dehydrogenase; short chain dehydrogenase/reductase family 12C, member 2; hydroxysteroid (17-beta) dehydrogenase 3; 17-beta-hydroxysteroid dehydrogenase type 3; 17-beta-HSD 3; Testicular 17-beta-hydroxysteroid dehydrogenase

HSD17B3; HSD17B3; EDH17B3; SDR12C2; EDH17B3; 17-beta-HSD 3

DHB3_HUMAN

Goat

310

No cross-reaction with other factors.

Store all reagents at 2-8 degree C.

Samples: Goat serum, plasma or Cell Culture Supernatant and organizations in the natural and recombinant Testosterone concentration. Assay Type: Sandwich. Detection Range: 10 ng/ml-0.156 ng/ml. Sensitivity: 0.05 ng/ml.

Intra-assay Precision: <= 8%. Inter-assay Precision: <= 12%

Principle of the assay: This experiment use double-sandwich elisa technique and the ELISA Kit provided is typical. The pre-coated antibody is Goat Testosterone monoclonal antibody and the detecting antibody is polyclonal antibody with biotin labeled. Samples and biotin labeling antibody are added into ELISA plate wells and washed out with PBS or TBS. Then Avidin-peroxidase conjugates are added to ELISA wells in order; Use TMB substrate for coloring after reactant thoroughly washed out by PBS or TBS. TMB turns into blue in peroxidase catalytic and finally turns into yellow under the action of acid. The color depth and the testing factors in samples are positively correlated.

4557649

NP_000188.1

NM_000197.1

34,516 Da

Androgen Biosynthesis Pathway (106154); Fatty Acyl-CoA Biosynthesis Pathway (106115); Fatty Acid, Triacylglycerol, And Ketone Body Metabolism Pathway (160977); Metabolic Pathways (132956); Metabolism Pathway (477135); Metabolism Of Lipids And Lipoproteins Pathway (160976); Metabolism Of Steroid Hormones And Vitamin D Pathway (106150); Prostate Cancer Pathway (755440); Steroid Biosynthesis Pathway (198866); Steroid Hormone Biosynthesis Pathway (82940)

This isoform of 17 beta-hydroxysteroid dehydrogenase is expressed predominantly in the testis and catalyzes the conversion of androstenedione to testosterone. It preferentially uses NADP as cofactor. Deficiency can result in male pseudohermaphroditism with gynecomastia. [provided by RefSeq, Jul 2008]

HSD17B3: Favors the reduction of androstenedione to testosterone. Uses NADPH while the two other EDH17B enzymes use NADH. Defects in HSD17B3 are the cause of male pseudohermaphrodism with gynecomastia (MPH). These individuals have unambiguous female external genitalia at birth, but fail to menstruate at the time of expected puberty and instead virilize as evidenced by growth of the phallus. Breast development may or may not take place. Belongs to the short-chain dehydrogenases/reductases (SDR) family. 17-beta-HSD 3 subfamily. Protein type: EC 1.1.1.64; Lipid Metabolism - androgen and estrogen; Oxidoreductase. Chromosomal Location of Human Ortholog: 9q22. Cellular Component: endoplasmic reticulum membrane; intracellular membrane-bound organelle. Molecular Function: testosterone 17-beta-dehydrogenase (NADP+) activity. Biological Process: steroid metabolic process; male genitalia development; androgen biosynthetic process. Disease: 17-beta Hydroxysteroid Dehydrogenase Iii Deficiency

48-Strip-Wells

315 USD

96-Strip-Wells

490