ELISA/assay

Human anti-Q-Ab ELISA Kit

MBS268488

48-Strip-Wells

250 USD

ELISA Kit

Human anti-Q-Ab ELISA Kit

[anti-Q-Ab]

[angiopoietin-2; Angiopoietin-2; angiopoietin-2; angiopoietin 2]

[Q-Ab]

[Angpt2; Angpt2; Ang2; Agpt2; Ang-2; Agpt2; ANG-2]

ANGP2_MOUSE

Human

496

No cross-reaction with other factors.

Store all reagents at 2-8 degree C.

Typical Standard Curve/Testing Data

Samples: Serum, plasma or cell culture supernatant. Assay Type: Quantitative Sandwich. Detection Range: 20 ng/ml-0.312ng/ml. Sensitivity: Up to 0.06 ng/ml.

Intra-assay Precision: <= 8%. Inter-assay Precision: <= 12%

Principle of the Assay: This experiment use double-sandwich elisa technique and the ELISA Kit provided is typical. The pre-coated antigen is Human anti-Q-Ab antigen.Samples and antigen are added into ELISA plate wells and washed out with PBS or TBS. Then Avidin-peroxidase conjugates are added to ELISA wells in order; Use TMB substrate for coloring after reactant thoroughly washed out by PBS or TBS. TMB turns into blue in peroxidase catalytic and finally turns into yellow under the action of acid. The color depth and the testing factors in samples are positively correlated.

31982508

NP_031452.2

NM_007426.4

56,576 Da

Cell Surface Interactions At The Vascular Wall Pathway (1001323); HIF-1 Signaling Pathway (695223); Hemostasis Pathway (1001295); PI3K-Akt Signaling Pathway (692242); PI3K-Akt Signaling Pathway (692979); Rap1 Signaling Pathway (869018); Rap1 Signaling Pathway (878042); Ras Signaling Pathway (869019); Tie2 Signaling Pathway (1001324)

This gene encodes an endothelial cell (EC)-derived regulator of angiogenesis and ligand for endothelial-specific receptor tyrosine kinase. The encoded protein acts as an anti-apoptotic factor for stressed ECs and a proapoptotic factor for resting ECs. [provided by RefSeq, Jan 2013]

ANGPT2: Binds to TEK/TIE2, competing for the ANGPT1 binding site, and modulating ANGPT1 signaling. Can induce tyrosine phosphorylation of TEK/TIE2 in the absence of ANGPT1. In the absence of angiogenic inducers, such as VEGF, ANGPT2-mediated loosening of cell-matrix contacts may induce endothelial cell apoptosis with consequent vascular regression. In concert with VEGF, it may facilitate endothelial cell migration and proliferation, thus serving as a permissive angiogenic signal. Interacts with TEK/TIE2, competing for the same binding site as ANGPT1. 3 isoforms of the human protein are produced by alternative splicing. Protein type: Secreted; Secreted, signal peptide. Cellular Component: extracellular space; cell projection; plasma membrane; extracellular region; nucleus. Molecular Function: protein binding; metal ion binding; receptor tyrosine kinase binding; vascular endothelial growth factor receptor binding. Biological Process: Tie receptor signaling pathway; multicellular organismal development; negative regulation of blood vessel endothelial cell migration; regulation of angiogenesis; negative regulation of positive chemotaxis; negative regulation of angiogenesis; positive regulation of angiogenesis; blood vessel morphogenesis; hemopoiesis; blood vessel remodeling; angiogenesis; cell differentiation; transmembrane receptor protein tyrosine kinase signaling pathway; endoderm development

48-Strip-Wells

250 USD

96-Strip-Wells

365

5x96-Strip-Wells

1480

10x96-Strip-Wells

2620