ELISA/assay

Chicken Ceruloplasmin (CP) ELISA Kit

MBS268435

48-Strip-Wells

275 USD

ELISA Kit

Chicken Ceruloplasmin (CP) ELISA Kit

[Ceruloplasmin (CP)]

[ceruloplasmin; Ceruloplasmin; ceruloplasmin; ceruloplasmin (ferroxidase); Ferroxidase]

[CP]

[CP; CP; CP-2]

CERU_HUMAN

Chicken

1065

No cross-reaction with other factors.

Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

Samples: Chicken Serum, Plasma Or Cell Culture Supernatant And Organizations In The Natural And Recombinant Col6 Concentration. Assay Type: Sandwich. Detection Range: 20 ng/ml-0.312 ng/ml. Sensitivity: 0.06 ng/ml.

Intra-assay Precision: <= 8%. Inter-assay Precision: <= 12%

Principle of the Assay: This experiment use double-sandwich elisa technique and the ELISA Kit provided is typical. The pre-coated antibody is Chicken COL6 monoclonal antibody and the detecting antibody is polyclonal antibody with biotin labeled. Samples and biotin labeling antibody are added into ELISA plate wells and washed out with PBS or TBS. Then Avidin-peroxidase conjugates are added to ELISA wells in order; Use TMB substrate for coloring after reactant thoroughly washed out by PBS or TBS. TMB turns into blue in peroxidase catalytic and finally turns into yellow under the action of acid. The color depth and the testing factors in samples are positively correlated.

4557485

NP_000087.1

NM_000096.3

122,205 Da

HIF-1-alpha Transcription Factor Network Pathway (138045); Iron Uptake And Transport Pathway (187191); Metal Ion SLC Transporters Pathway (161066); Porphyrin And Chlorophyll Metabolism Pathway (83021); Porphyrin And Chlorophyll Metabolism Pathway (407); SLC-mediated Transmembrane Transport Pathway (119558); Transmembrane Transport Of Small Molecules Pathway (106572); Transport Of Glucose And Other Sugars, Bile Salts And Organic Acids, Metal Ions And Amine Compounds Pathway (119571)

The protein encoded by this gene is a metalloprotein that binds most of the copper in plasma and is involved in the peroxidation of Fe(II)transferrin to Fe(III) transferrin. Mutations in this gene cause aceruloplasminemia, which results in iron accumulation and tissue damage, and is associated with diabetes and neurologic abnormalities. Two transcript variants, one protein-coding and the other not protein-coding, have been found for this gene. [provided by RefSeq, Feb 2012]

CP: Ceruloplasmin is a blue, copper-binding (6-7 atoms per molecule) glycoprotein. It has ferroxidase activity oxidizing Fe(2+) to Fe(3+) without releasing radical oxygen species. It is involved in iron transport across the cell membrane. Provides Cu(2+) ions for the ascorbate-mediated deaminase degradation of the heparan sulfate chains of GPC1. May also play a role in fetal lung development or pulmonary antioxidant defense. Defects in CP are the cause of aceruloplasminemia (ACERULOP). It is an autosomal recessive disorder of iron metabolism characterized by iron accumulation in the brain as well as visceral organs. Clinical features consist of the triad of retinal degeneration, diabetes mellitus and neurological disturbances. Ceruloplasmin levels are decreased in Wilson disease, in which copper cannot be incorporated into ceruloplasmin in liver because of defects in the copper-transporting ATPase 2. Belongs to the multicopper oxidase family. Protein type: Oxidoreductase; Cofactor and Vitamin Metabolism - porphyrin and chlorophyll; Secreted, signal peptide; Secreted; EC 1.16.3.1. Chromosomal Location of Human Ortholog: 3q23-q25. Cellular Component: extracellular space; lysosomal membrane; extracellular region. Molecular Function: ferroxidase activity; copper ion binding; chaperone binding. Biological Process: cellular iron ion homeostasis; copper ion transport; transmembrane transport. Disease: Aceruloplasminemia

48-Strip-Wells

275 USD

96-Strip-Wells

435

5x96-Strip-Wells

1755

10x96-Strip-Wells

3150