catalog number :
MBS268428
products type :
ELISA Kit
products full name :
Mouse Leukotriene B4 (LTB4) ELISA Kit
products short name :
Leukotriene B4 (LTB4)
other names :
leukotriene B4 receptor 2; Leukotriene B4 receptor 2; leukotriene B4 receptor 2; leukotriene B4 receptor BLT2; leukotriene B4 receptor 2; Leukotriene B4 receptor BLT2
products gene name :
LTB4
other gene names :
Ltb4r2; Ltb4r2; BLT2; LTB4-R2; LTB4-R 2; LTB4-R-2; 5830462O07Rik; Blt2; LTB4-R 2; LTB4-R2
uniprot entry name :
LT4R2_MOUSE
specificity :
No cross-reaction with other factors.
storage stability :
Store all reagents at 2-8 degree C.
other info1 :
Samples: Mouse serum, plasma or cell culture supernatant and organizations in the natural and recombinant LTB4 concentration. Assay Type: Sandwich. Detection Range: 20 ng/ml-0.312 ng/ml. Sensitivity: 0.06 ng/ml.
other info2 :
Intra-assay Precision: <= 8%. Inter-assay Precision: <= 12%
products description :
Principle of the Assay This experiment use double-sandwich elisa technique and the ELISA Kit provided is typical. The pre-coated antibody is Mouse LTB4 monoclonal antibody and the detecting antibody is polyclonal antibody with biotin labeled. Samples and biotin labeling antibody are added into ELISA plate wells and washed out with PBS or TBS. Then Avidin-peroxidase conjugates are added to ELISA wells in order; Use TMB substrate for coloring after reactant thoroughly washed out by PBS or TBS. TMB turns into blue in peroxidase catalytic and finally turns into yellow under the action of acid. The color depth and the testing factors in samples are positively correlated.
ncbi acc num :
NP_065236.1
ncbi gb acc num :
NM_020490.2
ncbi mol weight :
37,867 Da
ncbi pathways :
Calcium Signaling Pathway (83247); Calcium Signaling Pathway (459); Class A/1 (Rhodopsin-like Receptors) Pathway (1000942); Eicosanoid Ligand-binding Receptors Pathway (1000957); G Alpha (q) Signalling Events Pathway (1000975); GPCR Downstream Signaling Pathway (1000971); GPCR Ligand Binding Pathway (1000941); GPCRs, Other Pathway (198282); Gastrin-CREB Signalling Pathway Via PKC And MAPK (970815); Leukotriene Receptors Pathway (1000959)
uniprot summary :
LTB4R2: Low-affinity receptor for leukotrienes including leukotriene B4. Mediates chemotaxis of granulocytes and macrophages. The response is mediated via G-proteins that activate a phosphatidylinositol-calcium second messenger system. The rank order of affinities for the leukotrienes is LTB4 12-epi-LTB4 LTB5 LTB3. Belongs to the G-protein coupled receptor 1 family. Protein type: Membrane protein, integral; Membrane protein, multi-pass; Receptor, GPCR; Motility/polarity/chemotaxis; GPCR, family 1. Cellular Component: membrane; integral to membrane; plasma membrane. Molecular Function: G-protein coupled receptor activity; leukotriene B4 receptor activity; signal transducer activity; leukotriene receptor activity. Biological Process: G-protein coupled receptor protein signaling pathway; signal transduction; keratinocyte migration