ELISA/assay

Human Mucin 5 Subtype B (MUC5B) ELISA Kit

MBS268373

48-Strip-Wells

275 USD

ELISA Kit

Human Mucin 5 Subtype B (MUC5B) ELISA Kit

Mucin 5 Subtype B (MUC5B)

mucin-5B; Mucin-5B; mucin-5B; cervical mucin MUC5B; sublingual gland mucin; mucin 5, subtype B, tracheobronchial; high molecular weight salivary mucin MG1; mucin 5B, oligomeric mucus/gel-forming; Cervical mucin; High molecular weight salivary mucin MG1; Mucin-5 subtype B, tracheobronchial; Sublingual gland mucin

MUC5B

MUC5B; MUC5B; MG1; MUC5; MUC9; MUC-5B; MUC5; MUC-5B

MUC5B_HUMAN

Human

5762

No cross-reaction with other factors.

Store all reagents at 2-8 degree C.

Samples: Human serum, plasma or cell culture supernatant and organizations in the natural and recombinant MUC5B concentration. Assay Type: Sandwich. Detection Range: 20 ng/ml-0.312 ng/ml. Sensitivity: 0.06ng/ml.

Intra-assay Precision: <= 8%. Inter-assay Precision: <= 12%

Principle of the Assay This experiment use double-sandwich elisa technique and the ELISA Kit provided is typical. The pre-coated antibody is human MUC5B monoclonal antibody and the detecting antibody is polyclonal antibody with biotin labeled. Samples and biotin labeling antibody are added into ELISA plate wells and washed out with PBS or TBS. Then Avidin-peroxidase conjugates are added to ELISA wells in order; Use TMB substrate for coloring after reactant thoroughly washed out by PBS or TBS. TMB turns into blue in peroxidase catalytic and finally turns into yellow under the action of acid. The color depth and the testing factors in samples are positively correlated.

301172750

NP_002449.2

NM_002458.2

596,340 Da

Metabolism Of Proteins Pathway (106230); O-linked Glycosylation Pathway (980459); O-linked Glycosylation Of Mucins Pathway (530747); Post-translational Protein Modification Pathway (161012); Salivary Secretion Pathway (153376); Salivary Secretion Pathway (153352); Termination Of O-glycan Biosynthesis Pathway (530748)

This gene encodes a member of the mucin family of proteins, which are highly glycosylated macromolecular components of mucus secretions. This family member is the major gel-forming mucin in mucus. It is a major contributor to the lubricating and viscoelastic properties of whole saliva, normal lung mucus and cervical mucus. This gene has been found to be up-regulated in some human diseases, including sinus mucosa of chronic rhinosinusitis (CRS), CRS with nasal polyposis, chronic obstructive pulmonary disease (COPD) and H. pylori-associated gastric disease, and it may be involved in the pathogenesis of these diseases. [provided by RefSeq, Jul 2010]

MUC5B: Gel-forming mucin that is thought to contribute to the lubricating and viscoelastic properties of whole saliva and cervical mucus. Defects in MUC5B are a cause of susceptibility to pulmonary fibrosis idiopathic (IPF). Pulmonary fibrosis is a lung disease characterized by shortness of breath, radiographically evident diffuse pulmonary infiltrates, and varying degrees of inflammation and fibrosis on biopsy. It results in acute lung injury with subsequent scarring and endstage lung disease. A common polymorphism in the promoter of MUC5B is associated with familial interstitial pneumonia and idiopathic pulmonary fibrosis, suggesting that dysregulated MUC5B expression in the lung may be involved in the pathogenesis of pulmonary fibrosis (PubMed:21506741). Protein type: Secreted; Cell adhesion; Secreted, signal peptide. Chromosomal Location of Human Ortholog: 11p15.5. Cellular Component: extracellular space; Golgi lumen. Molecular Function: protein binding. Biological Process: protein amino acid O-linked glycosylation; cellular protein metabolic process; O-glycan processing; regulation of macrophage activation; defense response to bacterium; post-translational protein modification. Disease: Pulmonary Fibrosis, Idiopathic

48-Strip-Wells

275 USD

96-Strip-Wells

420