catalog number :
MBS268356
products type :
ELISA Kit
products full name :
Mouse 17beta-Estradiol ELISA Kit
products short name :
17beta-Estradiol
products name syn :
Mouse 17b-Estradiol ELISA Kit
other names :
17beta-estradiol dehydrogenase; 17beta-estradiol dehydrogenase; peroxisomal multifunctional enzyme type 2; 17beta-estradiol dehydrogenase; 17beta-estradiol dehydrogenase
other gene names :
HSD17B4
uniprot entry name :
Q28956_PIG
specificity :
No cross-reaction with other factors.
storage stability :
Store all reagents at 2-8 degree C.
other info1 :
Samples: Mouse serum, plasma or Cell Culture Supernatant and organizations in the natural and recombinant 17beta-Estradiol concentration. Assay Type: Sandwich. Detection Range: 20 ng/ml-0.312 ng/ml. Sensitivity: 0.06 ng/ml.
other info2 :
Intra-assay Precision: <= 8%. Inter-assay Precision: <= 12%
products description :
Principle of the assay: This experiment use double-sandwich elisa technique and the ELISA Kit provided is typical. The pre-coated antibody is Mouse 17beta-Estradiol monoclonal antibody and the detecting antibody is polyclonal antibody with biotin labeled. Samples and biotin labeling antibody are added into ELISA plate wells and washed out with PBS or TBS. Then Avidin-peroxidase conjugates are added to ELISA wells in order; Use TMB substrate for coloring after reactant thoroughly washed out by PBS or TBS. TMB turns into blue in peroxidase catalytic and finally turns into yellow under the action of acid. The color depth and the testing factors in samples are positively correlated.
ncbi acc num :
CAA55037.1
ncbi mol weight :
80,102 Da
ncbi pathways :
Metabolic Pathways (132936); Peroxisome Pathway (131215); Peroxisome Pathway (131126); Primary Bile Acid Biosynthesis Pathway (84373); Primary Bile Acid Biosynthesis Pathway (299)