Guinea pig urokinase plasminogen activator (uPA) ELISA Kit | MyBioSource MBS268314 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Guinea pig urokinase plasminogen activator (uPA) ELISA Kit

catalog :

MBS268314

quantity :

48-Strip-Wells

price :

315 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS268314

products type :

ELISA Kit

products full name :

Guinea pig urokinase plasminogen activator (uPA) ELISA Kit

products short name :

urokinase plasminogen activator (uPA)

other names :

urokinase plasminogen activator, partial; Urokinase-type plasminogen activator; urokinase-type plasminogen activator; U-plasminogen activator; plasminogen activator, urinary; plasminogen activator, urokinase

products gene name :

uPA

other gene names :

PLAU; PLAU; ATF; QPD; UPA; URK; u-PA; BDPLT5; U-plasminogen activator; uPA

uniprot entry name :

UROK_HUMAN

reactivity :

Guinea Pig

sequence length :

411

specificity :

No cross-reaction with other factors.

storage stability :

Store all reagents at 2-8 degree C.

other info1 :

Samples: Guinea pig serum, plasma or Cell Culture Supernatant and organizations in the natural and recombinant uPA concentration. Assay Type: Sandwich. Detection Range: 1000 pg/ml-15.6pg/ml. Sensitivity: 5 pg/ml.

other info2 :

Intra-assay Precision: <= 8%. Inter-assay Precision: <= 12%

products description :

Principle of the assay: This experiment use double-sandwich elisa technique and the ELISA Kit provided is typical. The pre-coated antibody is Guinea pig uPA monoclonal antibody and the detecting antibody is polyclonal antibody with biotin labeled. Samples and biotin labeling antibody are added into ELISA plate wells and washed out with PBS or TBS. Then Avidin-peroxidase conjugates are added to ELISA wells in order; Use TMB substrate for coloring after reactant thoroughly washed out by PBS or TBS. TMB turns into blue in peroxidase catalytic and finally turns into yellow under the action of acid. The color depth and the testing factors in samples are positively correlated.

ncbi gi num :

56068043

ncbi acc num :

AAV70488.1

ncbi mol weight :

46,908 Da

ncbi pathways :

ATF-2 Transcription Factor Network Pathway (138006); Blood Clotting Cascade Pathway (198840); Complement And Coagulation Cascades Pathway (198880); Complement And Coagulation Cascades Pathway (83073); Complement And Coagulation Cascades Pathway (484); DNA Damage Response (only ATM Dependent) Pathway (198827); Dissolution Of Fibrin Clot Pathway (106061); E2F Transcription Factor Network Pathway (137934); Endochondral Ossification Pathway (198812); FGF Signaling Pathway (137989)

ncbi summary :

This gene encodes a serine protease involved in degradation of the extracellular matrix and possibly tumor cell migration and proliferation. A specific polymorphism in this gene may be associated with late-onset Alzheimer's disease and also with decreased affinity for fibrin-binding. This protein converts plasminogen to plasmin by specific cleavage of an Arg-Val bond in plasminogen. Plasmin in turn cleaves this protein at a Lys-Ile bond to form a two-chain derivative in which a single disulfide bond connects the amino-terminal A-chain to the catalytically active, carboxy-terminal B-chain. This two-chain derivative is also called HMW-uPA (high molecular weight uPA). HMW-uPA can be further processed into LMW-uPA (low molecular weight uPA) by cleavage of chain A into a short chain A (A1) and an amino-terminal fragment. LMW-uPA is proteolytically active but does not bind to the uPA receptor. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Feb 2009]

uniprot summary :

uPA: Specifically cleave the zymogen plasminogen to form the active enzyme plasmin. Defects in PLAU are the cause of Quebec platelet disorder (QPD). QPD is an autosomal dominant bleeding disorder due to a gain-of-function defect in fibrinolysis. Although affected individuals do not exhibit systemic fibrinolysis, they show delayed onset bleeding after challenge, such as surgery. The hallmark of the disorder is markedly increased PLAU levels within platelets, which causes intraplatelet plasmin generation and secondary degradation of alpha-granule proteins. Belongs to the peptidase S1 family. 2 isoforms of the human protein are produced by alternative splicing. Protein type: Secreted; Secreted, signal peptide; Protease; Motility/polarity/chemotaxis; EC 3.4.21.73. Chromosomal Location of Human Ortholog: 10q22.2. Cellular Component: extracellular space; focal adhesion; cell surface; plasma membrane; extracellular region. Molecular Function: protein binding; serine-type endopeptidase activity. Biological Process: regulation of cell adhesion mediated by integrin; fibrinolysis; regulation of smooth muscle cell migration; smooth muscle cell migration; response to hypoxia; regulation of receptor activity; signal transduction; blood coagulation; chemotaxis; proteolysis; regulation of cell proliferation. Disease: Quebec Platelet Disorder; Alzheimer Disease

size1 :

48-Strip-Wells

price1 :

315 USD

size2 :

96-Strip-Wells

price2 :

490

more info or order :

MyBioSource product webpage