ELISA/assay

Mouse Endothelial Monocyte Activating Protein II (EMAP-II) ELISA Kit

MBS268308

48-Strip-Wells

290 USD

ELISA Kit

Mouse Endothelial Monocyte Activating Protein II (EMAP-II) ELISA Kit

Endothelial Monocyte Activating Protein II (EMAP-II)

endothelial-monocyte activating polypeptide II; Aminoacyl tRNA synthase complex-interacting multifunctional protein 1; aminoacyl tRNA synthase complex-interacting multifunctional protein 1; ARS-interacting multifunctional protein 1; endothelial monocyte-activating polypeptide 2; multisynthase complex auxiliary component p43; endothelial-monocyte activating polypeptide II; multisynthetase complex auxiliary component p43; small inducible cytokine subfamily E, member 1 (endothelial monocyte-activating); aminoacyl tRNA synthetase complex-interacting multifunctional protein 1; Multisynthase complex auxiliary component p43Cleaved into the following chain:Endothelial monocyte-activating polypeptide 2; EMAP-2Alternative name(s):Endothelial monocyte-activating polypeptide II; EMAP-II; Small inducible cytokine subfamily E member 1

EMAP-II

AIMP1; AIMP1; p43; HLD3; EMAP2; SCYE1; EMAPII; EMAP2; SCYE1; EMAP-2; EMAP-II

AIMP1_HUMAN

Mouse

312

No cross-reaction with other factors.

Store all reagents at 2-8 degree C.

Samples: Mouse serum, plasma or Cell Culture Supernatant and organizations in the natural and recombinant EMAP-II concentration. Assay Type: Sandwich. Detection Range: 20 ng/ml-0.312 ng/ml. Sensitivity: 0.06 ng/ml.

Intra-assay Precision: ? 8%. Inter-assay Precision: ? 12%

Principle of the assay: This experiment use double-sandwich elisa technique and the ELISA Kit provided is typical. The pre-coated antibody is Mouse EMAP-II monoclonal antibody and the detecting antibody is polyclonal antibody with biotin labeled. Samples and biotin labeling antibody are added into ELISA plate wells and washed out with PBS or TBS. Then Avidin-peroxidase conjugates are added to ELISA wells in order; Use TMB substrate for coloring after reactant thoroughly washed out by PBS or TBS. TMB turns into blue in peroxidase catalytic and finally turns into yellow under the action of acid. The color depth and the testing factors in samples are positively correlated.

498910

AAA62202.1

37,039 Da

Cytosolic TRNA Aminoacylation Pathway (105981); Gene Expression Pathway (105937); TRNA Aminoacylation Pathway (105980)

The protein encoded by this gene is a cytokine that is specifically induced by apoptosis, and it is involved in the control of angiogenesis, inflammation, and wound healing. The release of this cytokine renders the tumor-associated vasculature sensitive to tumor necrosis factor. The precursor protein is identical to the p43 subunit, which is associated with the multi-tRNA synthetase complex, and it modulates aminoacylation activity of tRNA synthetase in normal cells. This protein is also involved in the stimulation of inflammatory responses after proteolytic cleavage in tumor cells. Multiple transcript variants encoding different isoforms have been found for this gene. A pseudogene has been identified on chromosome 20. [provided by RefSeq, Dec 2008]

SCYE1 iso2: Non-catalytic component of the multisynthase complex. Stimulates the catalytic activity of cytoplasmic arginyl-tRNA synthase. Binds tRNA. Possesses inflammatory cytokine activity. Negatively regulates TGF-beta signaling through stabilization of SMURF2 by binding to SMURF2 and inhibiting its SMAD7-mediated degradation. Involved in glucose homeostasis through induction of glucagon secretion at low glucose levels. Promotes dermal fibroblast proliferation and wound repair. Regulates KDELR1- mediated retention of HSP90B1/gp96 in the endoplasmic reticulum. Plays a role in angiogenesis by inducing endothelial cell migration at low concentrations and endothelian cell apoptosis at high concentrations. Induces maturation of dendritic cells and monocyte cell adhesion. Modulates endothelial cell responses by degrading HIF-1A through interaction with PSMA7. Defects in AIMP1 are the cause of leukodystrophy hypomyelinating type 3 (HLD3). A severe autosomal recessive hypomyelinating leukodystrophy characterized by early infantile onset of global developmental delay, lack of development, lack of speech acquisition, and peripheral spasticity associated with decreased myelination in the central nervous system. 2 isoforms of the human protein are produced by alternative splicing. Protein type: Motility/polarity/chemotaxis. Chromosomal Location of Human Ortholog: 4q24. Cellular Component: Golgi apparatus; extracellular space; aminoacyl-tRNA synthetase multienzyme complex; transport vesicle; cell surface; membrane; endoplasmic reticulum; cytoplasm; cytosol; nucleus. Molecular Function: methionine-tRNA ligase activity; protein binding; protein homodimerization activity; cytokine activity; tRNA binding. Biological Process: tRNA aminoacylation for protein translation; cell-cell signaling; apoptosis; negative regulation of endothelial cell proliferation; response to wounding; methionyl-tRNA aminoacylation; glucose metabolic process; gene expression; angiogenesis; cell adhesion; chemotaxis; signal transduction; inflammatory response; leukocyte migration. Disease: Leukodystrophy, Hypomyelinating, 3

48-Strip-Wells

290 USD

96-Strip-Wells

450