ELISA/assay

Human Interleukin 4 (IL-4) ELISA Kit

MBS268288

48-Strip-Wells

275 USD

ELISA Kit

Human Interleukin 4 (IL-4) ELISA Kit

Interleukin 4 (IL-4)

interleukin-4 isoform 1; Interleukin-4; interleukin-4; binetrakin; pitrakinra; B cell growth factor 1; interleukin 4 variant 2; B_cell stimulatory factor 1; lymphocyte stimulatory factor 1; interleukin 4; B-cell stimulatory factor 1; BSF-1; Binetrakin; Lymphocyte stimulatory factor 1; Pitrakinra

IL-4

IL4; IL4; BSF1; IL-4; BCGF1; BSF-1; BCGF-1; IL-4; BSF-1

IL4_HUMAN

Human

153

No cross-reaction with other factors.

Store all reagents at 2-8 degree C.

Samples: Human serum, plasma or Cell Culture Supernatant and organizations in the natural and recombinant IL-4 concentration. Assay Type: Sandwich. Detection Range: 1000 pg/ml-15.6 pg/ml. Sensitivity: 5 pg/ml.

Intra-assay Precision: <= 8%. Inter-assay Precision: <= 12%

Principle of the assay: This experiment use double-sandwich elisa technique and the ELISA Kit provided is typical. The pre-coated antibody is Human IL-4 monoclonal antibody and the detecting antibody is polyclonal antibody with biotin labeled. Samples and biotin labeling antibody are added into ELISA plate wells and washed out with PBS or TBS. Then Avidin-peroxidase conjugates are added to ELISA wells in order; Use TMB substrate for coloring after reactant thoroughly washed out by PBS or TBS. TMB turns into blue in peroxidase catalytic and finally turns into yellow under the action of acid. The color depth and the testing factors in samples are positively correlated.

4504669

NP_000580.1

NM_000589.3

15,797 Da

Allograft Rejection Pathway (83123); Allograft Rejection Pathway (535); Asthma Pathway (83120); Asthma Pathway (532); Autoimmune Thyroid Disease Pathway (83121); Autoimmune Thyroid Disease Pathway (533); CD40/CD40L Signaling Pathway (138061); Calcineurin-regulated NFAT-dependent Transcription In Lymphocytes Pathway (137993); Calcium Signaling In The CD4+ TCR Pathway (137941); Cytokine-cytokine Receptor Interaction Pathway (83051)

The protein encoded by this gene is a pleiotropic cytokine produced by activated T cells. This cytokine is a ligand for interleukin 4 receptor. The interleukin 4 receptor also binds to IL13, which may contribute to many overlapping functions of this cytokine and IL13. STAT6, a signal transducer and activator of transcription, has been shown to play a central role in mediating the immune regulatory signal of this cytokine. This gene, IL3, IL5, IL13, and CSF2 form a cytokine gene cluster on chromosome 5q, with this gene particularly close to IL13. This gene, IL13 and IL5 are found to be regulated coordinately by several long-range regulatory elements in an over 120 kilobase range on the chromosome. Two alternatively spliced transcript variants of this gene encoding distinct isoforms have been reported. [provided by RefSeq, Jul 2008]

IL4: Participates in at least several B-cell activation processes as well as of other cell types. It is a costimulator of DNA-synthesis. It induces the expression of class II MHC molecules on resting B-cells. It enhances both secretion and cell surface expression of IgE and IgG1. It also regulates the expression of the low affinity Fc receptor for IgE (CD23) on both lymphocytes and monocytes. Genetic variations in IL4 may be a cause of susceptibility to ischemic stroke (ISCHSTR); also known as cerebrovascular accident or cerebral infarction. A stroke is an acute neurologic event leading to death of neural tissue of the brain and resulting in loss of motor, sensory and/or cognitive function. Ischemic strokes, resulting from vascular occlusion, is considered to be a highly complex disease consisting of a group of heterogeneous disorders with multiple genetic and environmental risk factors. Belongs to the IL-4/IL-13 family. 2 isoforms of the human protein are produced by alternative splicing. Protein type: Motility/polarity/chemotaxis; Secreted, signal peptide; Cytokine; Cell cycle regulation; Secreted. Chromosomal Location of Human Ortholog: 5q31.1. Cellular Component: extracellular space; external side of plasma membrane. Molecular Function: protein binding; growth factor activity; cytokine activity; interleukin-4 receptor binding. Biological Process: regulation of isotype switching; positive regulation of isotype switching to IgG isotypes; negative regulation of osteoclast differentiation; positive regulation of transcription, DNA-dependent; microglial cell activation; regulation of proton transport; female pregnancy; chemotaxis; positive regulation of activated T cell proliferation; response to organic cyclic substance; positive regulation of interleukin-10 production; positive regulation of isotype switching to IgE isotypes; B cell costimulation; positive regulation of MHC class II biosynthetic process; connective tissue growth factor biosynthetic process; positive regulation of B cell proliferation; positive regulation of T cell proliferation; positive regulation of interleukin-13 production; negative regulation of macrophage activation; response to nutrient; response to drug; cholesterol metabolic process; negative regulation of chronic inflammatory response; regulation of immune response; T-helper 2 type immune response; regulation of phosphorylation; B cell activation; negative regulation of nitric oxide biosynthetic process; negative regulation of acute inflammatory response; defense response to protozoan; T-helper 1 cell lineage commitment; response to ethanol; positive regulation of chemokine biosynthetic process; positive regulation of tyrosine phosphorylation of Stat5 protein; innate immune response in mucosa; positive regulation of T cell differentiation; retina development in camera-type eye; response to cytokine stimulus; B cell differentiation; cellular defense response; positive regulation of transcription from RNA polymerase II promoter; immune response; positive regulation of transcription factor activity; T-helper 2 cell differentiation; negative regulation of transcription, DNA-dependent; positive regulation of defense response to virus by host; negative regulation of apoptosis. Disease: Stroke, Ischemic

48-Strip-Wells

275 USD

96-Strip-Wells

420