ELISA/assay

Mouse Gastrin Releasing Peptide (GRP) ELISA Kit

MBS268207

48-Strip-Wells

260 USD

ELISA Kit

Mouse Gastrin Releasing Peptide (GRP) ELISA Kit

[Gastrin Releasing Peptide (GRP)]

[gastrin-releasing peptide isoform 3 preproprotein; Gastrin-releasing peptide; gastrin-releasing peptide; bombesin; prepro-GRP; neuromedin C; pre-progastrin releasing peptide; gastrin-releasing peptide; GRP-10]

[GRP]

[GRP; GRP; BN; GRP-10; proGRP; preproGRP; GRP]

GRP_HUMAN

Mouse

138

No cross-reaction with other factors.

Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

Samples: Mouse Serum, Plasma Or Cell Culture Supernatant And Organizations In The Natural And Recombinant Gpi Concentration. Assay Type: Sandwich. Detection Range: 20 ng/ml-0.312 ng/ml. Sensitivity: 0.06 ng/ml.

Intra-assay Precision: <= 8%. Inter-assay Precision: <= 12%

Principle of the Assay: This experiment use double-sandwich elisa technique and the ELISA Kit provided is typical. The pre-coated antibody is Mouse GPI monoclonal antibody and the detecting antibody is polyclonal antibody with biotin labeled. Samples and biotin labeling antibody are added into ELISA plate wells and washed out with PBS or TBS. Then Avidin-peroxidase conjugates are added to ELISA wells in order; Use TMB substrate for coloring after reactant thoroughly washed out by PBS or TBS. TMB turns into blue in peroxidase catalytic and finally turns into yellow under the action of acid. The color depth and the testing factors in samples are positively correlated.

60498999

NP_001012531.1

NM_001012513.1

15,533 Da

Class A/1 (Rhodopsin-like Receptors) Pathway (106357); G Alpha (q) Signalling Events Pathway (106043); GPCR Downstream Signaling Pathway (119548); GPCR Ligand Binding Pathway (161020); Gastrin-CREB Signalling Pathway Via PKC And MAPK (645295); Incretin Synthesis, Secretion, And Inactivation Pathway (187170); Metabolism Of Proteins Pathway (106230); Peptide Hormone Metabolism Pathway (771603); Peptide Ligand-binding Receptors Pathway (106358); Signal Transduction Pathway (477114)

This gene encodes a member of the bombesin-like family of gastrin-releasing peptides. Its preproprotein, following cleavage of a signal peptide, is further processed to produce either the 27 aa gastrin-releasing peptide or the 10 aa neuromedin C. These smaller peptides regulate numerous functions of the gastrointestinal and central nervous systems, including release of gastrointestinal hormones, smooth muscle cell contraction, and epithelial cell proliferation. These peptides are also likely to play a role in human cancers of the lung, colon, stomach, pancreas, breast, and prostate. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jul 2008]

GRP: GRP stimulates gastrin release as well as other gastrointestinal hormones. Operates as a negative feedback regulating fear and established a causal relationship between GRP- receptor gene expression, long-term potentiation, and amygdala- dependent memory for fear. Belongs to the bombesin/neuromedin-B/ranatensin family. 3 isoforms of the human protein are produced by alternative splicing. Protein type: Secreted, signal peptide; Secreted. Chromosomal Location of Human Ortholog: 18q21.1-q21.32. Cellular Component: extracellular space; extracellular region. Molecular Function: neuropeptide hormone activity; receptor binding. Biological Process: neuropeptide signaling pathway; signal transduction

48-Strip-Wells

260 USD

96-Strip-Wells

405

5x96-Strip-Wells

1615

10x96-Strip-Wells

2880