catalog number :
MBS268195
products type :
ELISA Kit
products full name :
Human Myoglobin (MYO) ELISA Kit
products short name :
Myoglobin (MYO)
other names :
myoglobin; Myoglobin; myoglobin; myoglobin
other gene names :
MB; MB; PVALB; myoglobgin
uniprot entry name :
MYG_HUMAN
specificity :
No cross-reaction with other factors.
storage stability :
Store all reagents at 2-8 degree C.
other info1 :
Samples: Human serum, plasma or Cell Culture Supernatant and organizations in the natural and recombinant MYO concentration. Assay Type: Sandwich. Detection Range: 50 ng/ml-0.78 ng/ml. Sensitivity: 0.1 ng/ml.
other info2 :
Intra-assay Precision: <= 8%. Inter-assay Precision: <= 12%
products description :
Principle of the assay: This experiment use double-sandwich elisa technique and the ELISA Kit provided is typical. The pre-coated antibody is human MYO monoclonal antibody and the detecting antibody is polyclonal antibody with biotin labeled. Samples and biotin labeling antibody are added into ELISA plate wells and washed out with PBS or TBS. Then Avidin-peroxidase conjugates are added to ELISA wells in order; Use TMB substrate for coloring after reactant thoroughly washed out by PBS or TBS. TMB turns into blue in peroxidase catalytic and finally turns into yellow under the action of acid. The color depth and the testing factors in samples are positively correlated.
ncbi acc num :
NP_976312.1
ncbi gb acc num :
NM_203378.1
ncbi mol weight :
17,184 Da
ncbi summary :
This gene encodes a member of the globin superfamily and is expressed in skeletal and cardiac muscles. The encoded protein is a haemoprotein contributing to intracellular oxygen storage and transcellular facilitated diffusion of oxygen. At least three alternatively spliced transcript variants encoding the same protein have been reported. [provided by RefSeq, Jul 2008]
uniprot summary :
MB: Serves as a reserve supply of oxygen and facilitates the movement of oxygen within muscles. Belongs to the globin family. Protein type: Carrier. Chromosomal Location of Human Ortholog: 22q13.1. Molecular Function: iron ion binding; heme binding; oxygen binding; oxygen transporter activity. Biological Process: slow-twitch skeletal muscle fiber contraction; response to hydrogen peroxide; oxygen transport; heart development; brown fat cell differentiation; response to hypoxia; response to hormone stimulus; enucleate erythrocyte differentiation