catalog number :
MBS268081
products type :
ELISA Kit
products full name :
Rat 12-Hydroxyeicosatetraenoic Acid (12-HETE) ELISA Kit
products short name :
12-Hydroxyeicosatetraenoic Acid (12-HETE)
other names :
12-(S)-hydroxy-5,8,10,14-eicosatetraenoic acid receptor; 12-(S)-hydroxy-5,8,10,14-eicosatetraenoic acid receptor; 12-(S)-hydroxy-5,8,10,14-eicosatetraenoic acid receptor; 12-(S)-hydroxy-5,8,10,14-eicosatetraenoic acid receptor; 12-(S)-HETE receptor; 12-(S)-HETE acid receptor; G-protein coupled receptor 31; probable G-protein coupled receptor 31; bA517H2.2 (G protein-coupled receptor 31); hydroxyeicosatetraenoic (HETE) acid receptor 1; 12-(S)-hydroxy-5,8,10,14-eicosatetraenoic acid (HETE) receptor; G protein-coupled receptor 31; G-protein coupled receptor 31
products gene name :
12-HETE
other gene names :
GPR31; GPR31; HETER; HETER1; 12-HETER; 12-(S)-HETE receptor; 12-HETER
uniprot entry name :
GPR31_HUMAN
storage stability :
Store all reagents at 2-8 degree C.
other info1 :
Samples: This product is suitable for in vitro qualitative detection of Rat serum, plasma or cell culture supernatant and organizations in the natural and recombinant 12-HETE concentration. Detection Range: 100 ng/ml-1.56 ng/ml. Sensitivity: the minimum detectable Rat 12-HETE up to 0.5 ng/ml.
other info2 :
Intra-assay Precision: <= 8%. Inter-assay Precision: <= 12%
products description :
Principle of the Assay: This experiment use double-sandwich elisa technique and the ELISA Kit provided is typical. The pre-coated antibody is Rat 12-HETE monoclonal antibody and the detecting antibody is polyclonal antibody with biotin labeled. Samples and biotin labeling antibody are added into ELISA plate wells and washed out with PBS or TBS. Then Avidin-peroxidase conjugates are added to ELISA wells in order; Use TMB substrate for coloring after reactant thoroughly washed out by PBS or TBS. TMB turns into blue in peroxidase catalytic and finally turns into yellow under the action of acid. The color depth and the testing factors in samples are positively correlated.
ncbi acc num :
NP_005290.2
ncbi gb acc num :
NM_005299.2
ncbi mol weight :
35,075 Da
ncbi pathways :
Class A/1 (Rhodopsin-like Receptors) Pathway 106357!!Free Fatty Acid Receptors Pathway 161023!!GPCR Ligand Binding Pathway 161020!!GPCRs, Class A Rhodopsin-like Pathway 198886!!Signal Transduction Pathway 477114!!Signaling By GPCR Pathway 106356