catalog number :
MBS267959
products type :
ELISA Kit
products full name :
Mouse pancreatic amylase (PAMY) ELISA Kit
products short name :
pancreatic amylase (PAMY)
other names :
pancreatic alpha-amylase; Pancreatic alpha-amylase; pancreatic alpha-amylase; PA; pancreatic amylase; amylase 2, pancreatic; 1,4-alpha-D-glucan glucanohydrolase; amylase 2a3; 1,4-alpha-D-glucan glucanohydrolase
products gene name :
PAMY
other gene names :
Amy2a3; Amy2; Amy2; PA
uniprot entry name :
AMYP_RAT
specificity :
No cross-reaction with other factors.
storage stability :
Store all reagents at 2-8 degree C.
other info1 :
Samples: Mouse serum, plasma or Cell Culture Supernatant and organizations in the natural and recombinant PAMY concentration. Assay Type: Sandwich. Detection Range: 1000 U/L-15.6 U/L. Sensitivity: 5 U/L.
other info2 :
Intra-assay Precision: <= 8%. Inter-assay Precision: <= 12%
products description :
Principle of the assay: This experiment use double-sandwich elisa technique and the ELISA Kit provided is typical. The pre-coated antibody is Mouse PAMY monoclonal antibody and the detecting antibody is polyclonal antibody with biotin labeled. Samples and biotin labeling antibody are added into ELISA plate wells and washed out with PBS or TBS. Then Avidin-peroxidase conjugates are added to ELISA wells in order; Use TMB substrate for coloring after reactant thoroughly washed out by PBS or TBS. TMB turns into blue in peroxidase catalytic and finally turns into yellow under the action of acid. The color depth and the testing factors in samples are positively correlated.
ncbi acc num :
NP_113690.1
ncbi gb acc num :
NM_031502.1
ncbi mol weight :
57,177 Da
ncbi pathways :
Carbohydrate Digestion And Absorption Pathway (170716); Carbohydrate Digestion And Absorption Pathway (170654); Metabolic Pathways (132933); Starch And Sucrose Metabolism Pathway (83366); Starch And Sucrose Metabolism Pathway (344)
uniprot summary :
AMY2A: Amylases are secreted proteins that hydrolyze 1,4-alpha-glucoside bonds in oligosaccharides and polysaccharides, and thus catalyze the first step in digestion of dietary starch and glycogen. The human genome has a cluster of several amylase genes that are expressed at high levels in either salivary gland or pancreas. This gene encodes an amylase isoenzyme produced by the pancreas. [provided by RefSeq, Jul 2008]. Protein type: Carbohydrate Metabolism - starch and sucrose; Hydrolase; Secreted; Secreted, signal peptide; EC 3.2.1.1. Cellular Component: extracellular space. Molecular Function: cation binding; alpha-amylase activity; protein binding; calcium ion binding; chloride ion binding. Biological Process: carbohydrate catabolic process; carbohydrate metabolic process