ELISA/assay

Human Glycated albumin (GA) ELISA Kit

MBS267874

48-Strip-Wells

275 USD

ELISA Kit

Human Glycated albumin (GA) ELISA Kit

Glycated albumin (GA)

glutaminase liver isoform, mitochondrial isoform 4; Glutaminase liver isoform, mitochondrial; glutaminase liver isoform, mitochondrial; glutaminase I; breast cell glutaminase; L-glutamine amidohydrolase; phosphate-activated glutaminase; phosphate-dependent glutaminase; glutaminase 2 (liver, mitochondrial); L-glutaminase; L-glutamine amidohydrolase

GA

GLS2; GLS2; GA; GLS; LGA; hLGA; GA; GLS

GLSL_HUMAN

Human

337

No cross-reaction with other factors.

Store all reagents at 2-8 degree C.

Samples: Human serum, plasma or cell culture supernatant and organizations in the natural and recombinant GA concentration. Assay Type: Sandwich. Detection Range: 1000 ng/ml-15.6 ng/ml. Sensitivity: 5 ng/ml.

Intra-assay Precision: <= 8%. Inter-assay Precision: <= 12%

Principle of the Assay This experiment use double-sandwich elisa technique and the ELISA Kit provided is typical. The pre-coated antibody is human GA monoclonal antibody and the detecting antibody is polyclonal antibody with biotin labeled. Samples and biotin labeling antibody are added into ELISA plate wells and washed out with PBS or TBS. Then Avidin-peroxidase conjugates are added to ELISA wells in order; Use TMB substrate for coloring after reactant thoroughly washed out by PBS or TBS. TMB turns into blue in peroxidase catalytic and finally turns into yellow under the action of acid. The color depth and the testing factors in samples are positively correlated.

526118241

NP_001267726.1

NM_001280797.1

66,323 Da

Alanine, Aspartate And Glutamate Metabolism Pathway (101142); Alanine, Aspartate And Glutamate Metabolism Pathway (100063); Amino Acid Synthesis And Interconversion (transamination) Pathway (106173); Arginine And Proline Metabolism Pathway (82957); Arginine And Proline Metabolism Pathway (323); D-Glutamine And D-glutamate Metabolism Pathway (82971); D-Glutamine And D-glutamate Metabolism Pathway (340); GABAergic Synapse Pathway (377263); GABAergic Synapse Pathway (377129); Glutamate Neurotransmitter Release Cycle Pathway (106522)

The protein encoded by this gene is a mitochondrial phosphate-activated glutaminase that catalyzes the hydrolysis of glutamine to stoichiometric amounts of glutamate and ammonia. Originally thought to be liver-specific, this protein has been found in other tissues as well. Alternative splicing results in multiple transcript variants that encode different isoforms. [provided by RefSeq, Jul 2013]

GLS2: Plays an important role in the regulation of glutamine catabolism. Promotes mitochondrial respiration and increases ATP generation in cells by catalyzing the synthesis of glutamate and alpha-ketoglutarate. Increases cellular anti-oxidant function via NADH and glutathione production. May play a role in preventing tumor proliferation. Belongs to the glutaminase family. Protein type: Hydrolase; Other Amino Acids Metabolism - D-Glutamine and D-glutamate; Amino Acid Metabolism - arginine and proline; Amino Acid Metabolism - alanine, aspartate and glutamate; Energy Metabolism - nitrogen; EC 3.5.1.2; Mitochondrial. Chromosomal Location of Human Ortholog: 12q13.3. Cellular Component: mitochondrion; mitochondrial matrix. Molecular Function: protein binding; glutaminase activity. Biological Process: regulation of apoptosis; amino acid metabolic process; synaptic transmission; glutamate secretion; neurotransmitter secretion; glutamine metabolic process; amino acid biosynthetic process

48-Strip-Wells

275 USD

96-Strip-Wells

420