Mouse Peroxisome Proliferator Activated Receptor Gamma (PPAR-gamma) ELISA Kit | MyBioSource MBS267873 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Mouse Peroxisome Proliferator Activated Receptor Gamma (PPAR-gamma) ELISA Kit

catalog :

MBS267873

quantity :

48-Strip-Wells

price :

290 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS267873

products type :

ELISA Kit

products full name :

Mouse Peroxisome Proliferator Activated Receptor Gamma (PPAR-gamma) ELISA Kit

products short name :

Peroxisome Proliferator Activated Receptor Gamma (PPAR-gamma)

products name syn :

Mouse Peroxisome Proliferator Activated Receptor Gamma (PPAR-g) ELISA Kit

other names :

peroxisome proliferator activated receptor gamma; Peroxisome proliferator-activated receptor gamma; Nuclear receptor subfamily 1 group C member 3

products gene name :

PPAR-gamma

other gene names :

PPARG; NR1C3; PPAR-gamma

uniprot entry name :

PPARG_HUMAN

reactivity :

Mouse

sequence length :

477

specificity :

No cross-reaction with other factors.

storage stability :

Store all reagents at 2-8 degree C.

other info1 :

Samples: Mouse serum, plasma or Cell Culture Supernatant and organizations in the natural and recombinant PPAR-? concentration. Assay Type: Sandwich. Detection Range: 20 ng/ml-0.312ng/ml. Sensitivity: 0.06ng/ml.

other info2 :

Intra-assay Precision: ? 8%. Inter-assay Precision: ? 12%

products description :

Principle of the assay: This experiment use double-sandwich elisa technique and the ELISA Kit provided is typical. The pre-coated antibody is Mouse PPAR-? monoclonal antibody and the detecting antibody is polyclonal antibody with biotin labeled. Samples and biotin labeling antibody are added into ELISA plate wells and washed out with PBS or TBS. Then Avidin-peroxidase conjugates are added to ELISA wells in order; Use TMB substrate for coloring after reactant thoroughly washed out by PBS or TBS. TMB turns into blue in peroxidase catalytic and finally turns into yellow under the action of acid. The color depth and the testing factors in samples are positively correlated.

ncbi gi num :

17978516

ncbi acc num :

AAA80314.2

ncbi mol weight :

21,580 Da

uniprot summary :

PPAR-gamma: a transcription factor, member of the nuclear hormone receptor superfamily. Receptor for hypolipidemic drugs and fatty acids. Preferentially expressed in adipocytes as well as in vascular smooth muscle cells and macrophage. Regulator of adipogenesis and lipid metabolism, modulates insulin sensitivity, cell proliferation and inflammation. Phosphorylated and inhibited by MAP kinase. Heterodimerizes with the retinoid X receptor. Interacts with NCOA6 coactivator, leading to a strong increase in transcription of target genes. Two splice-variant isoforms have been described. Protein type: Nuclear receptor; DNA-binding. Chromosomal Location of Human Ortholog: 3p25. Cellular Component: nucleoplasm; perinuclear region of cytoplasm; cytosol; nucleus. Molecular Function: ligand-dependent nuclear receptor activity; transcription activator binding; zinc ion binding; drug binding; alpha-actinin binding; protein phosphatase binding; retinoid X receptor binding; arachidonic acid binding; protein binding; enzyme binding; DNA binding; ligand-dependent nuclear receptor transcription coactivator activity; prostaglandin receptor activity; sequence-specific DNA binding; steroid hormone receptor activity; estrogen receptor binding; chromatin binding; transcription factor activity. Biological Process: heart development; negative regulation of collagen biosynthetic process; positive regulation of transcription, DNA-dependent; cell maturation; rhythmic process; lipid homeostasis; glucose homeostasis; response to lipid; response to vitamin A; response to caffeine; positive regulation of oligodendrocyte differentiation; placenta development; long-chain fatty acid transport; organ regeneration; cell fate commitment; monocyte differentiation; negative regulation of acute inflammatory response; regulation of circadian rhythm; response to starvation; negative regulation of telomerase activity; cellular response to insulin stimulus; response to mechanical stimulus; lipoprotein transport; response to estrogen stimulus; brown fat cell differentiation; positive regulation of fat cell differentiation; positive regulation of transcription factor activity; steroid hormone mediated signaling; positive regulation of transcription from RNA polymerase II promoter; fatty acid oxidation; negative regulation of transcription, DNA-dependent; positive regulation of phagocytosis, engulfment; negative regulation of smooth muscle cell proliferation; low-density lipoprotein receptor biosynthetic process; negative regulation of transcription from RNA polymerase II promoter; signal transduction; epithelial cell differentiation; regulation of blood pressure; response to nutrient; caspase activation; transcription initiation from RNA polymerase II promoter; response to retinoic acid; G-protein coupled receptor protein signaling pathway; response to low density lipoprotein stimulus; white fat cell differentiation; positive regulation of fatty acid oxidation; innate immune response; gene expression; lipid metabolic process; response to cold; negative regulation of cell growth. Disease: Obesity; Carotid Intimal Medial Thickness 1; Lipodystrophy, Familial Partial, Type 3; Diabetes Mellitus, Noninsulin-dependent

size1 :

48-Strip-Wells

price1 :

290 USD

size2 :

96-Strip-Wells

price2 :

450

more info or order :

MyBioSource product webpage