ELISA/assay

Human Thromboxane A2 (TX-A2) ELISA Kit

MBS267848

48-Strip-Wells

275 USD

ELISA Kit

Human Thromboxane A2 (TX-A2) ELISA Kit

Thromboxane A2 (TX-A2)

thromboxane A2 receptor isoform beta; Thromboxane A2 receptor; thromboxane A2 receptor; prostanoid TP receptor; thromboxane A2 receptor; Prostanoid TP receptor

TX-A2

TBXA2R; TBXA2R; TXA2-R; BDPLT13; TXA2-R

TA2R_HUMAN

Human

407

No cross-reaction with other factors.

Store all reagents at 2-8 degree C.

Samples: Human serum, plasma or Cell Culture Supernatant and organizations in the natural and recombinant TX-A2 concentration. Assay Type: Sandwich. Detection Range: 1000 pg/ml-15.6 pg/ml. Sensitivity: 5 pg/ml.

Intra-assay Precision: <= 8%. Inter-assay Precision: <= 12%

Principle of the assay: This experiment use double-sandwich elisa technique and the ELISA Kit provided is typical. The pre-coated antibody is human TX-A2 monoclonal antibody and the detecting antibody is polyclonal antibody with biotin labeled. Samples and biotin labeling antibody are added into ELISA plate wells and washed out with PBS or TBS. Then Avidin-peroxidase conjugates are added to ELISA wells in order; Use TMB substrate for coloring after reactant thoroughly washed out by PBS or TBS. TMB turns into blue in peroxidase catalytic and finally turns into yellow under the action of acid. The color depth and the testing factors in samples are positively correlated.

260593681

NP_963998.2

NM_201636.2

44,205 Da

Calcium Signaling Pathway (83050); Calcium Signaling Pathway (459); Class A/1 (Rhodopsin-like Receptors) Pathway (106357); Eicosanoid Ligand-binding Receptors Pathway (106370); G Alpha (12/13) Signalling Events Pathway (106041); G Alpha (q) Signalling Events Pathway (106043); GPCR Downstream Signaling Pathway (119548); GPCR Ligand Binding Pathway (161020); GPCRs, Class A Rhodopsin-like Pathway (198886); Gastrin-CREB Signalling Pathway Via PKC And MAPK (645295)

This gene encodes a member of the G protein-coupled receptor family. The protein interacts with thromboxane A2 to induce platelet aggregation and regulate hemostasis. A mutation in this gene results in a bleeding disorder. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2009]

TXA2-R: Receptor for thromboxane A2 (TXA2), a potent stimulator of platelet aggregation. The activity of this receptor is mediated by a G-protein that activates a phosphatidylinositol-calcium second messenger system. In the kidney, the binding of TXA2 to glomerular TP receptors causes intense vasoconstriction. Activates phospholipase C. Isoform 1 activates adenylyl cyclase. Isoform 2 inhibits adenylyl cyclase. Defects in TBXA2R are the cause of susceptibility to bleeding disorder platelet-type 13 (BDPLT13). BDPLT13 is a disorder characterized by reduced platelet aggregation and a tendency to mild mucocutaneous bleeding. Belongs to the G-protein coupled receptor 1 family. 2 isoforms of the human protein are produced by alternative splicing. Protein type: Membrane protein, integral; Membrane protein, multi-pass; Receptor, GPCR; GPCR, family 1. Chromosomal Location of Human Ortholog: 19p13.3. Cellular Component: integral to plasma membrane; plasma membrane; acrosome. Molecular Function: protein binding; guanyl-nucleotide exchange factor activity; thromboxane A2 receptor activity. Biological Process: response to drug; second-messenger-mediated signaling; platelet activation; response to lipopolysaccharide; G-protein coupled receptor protein signaling pathway; positive regulation of angiogenesis; elevation of cytosolic calcium ion concentration; positive regulation of vasoconstriction; positive regulation of smooth muscle contraction; blood coagulation; inflammatory response; response to nutrient; positive regulation of blood pressure; positive regulation of GTPase activity

48-Strip-Wells

275 USD

96-Strip-Wells

420