Human Glutathione S Transferase Pi (GSTp) ELISA Kit | MyBioSource MBS267722 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Human Glutathione S Transferase Pi (GSTp) ELISA Kit

catalog :

MBS267722

quantity :

48-Strip-Wells

price :

275 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS267722

products type :

ELISA Kit

products full name :

Human Glutathione S Transferase Pi (GSTp) ELISA Kit

products short name :

Glutathione S Transferase Pi (GSTp)

other names :

glutathione S-transferase pi; Glutathione S-transferase P; glutathione S-transferase P; GSTP1-1; GST class-pi; deafness, X-linked 7; epididymis secretory protein Li 22; fatty acid ethyl ester synthase III; glutathione S-transferase pi 1; GST class-pi; GSTP1-1

products gene name :

GSTp

other gene names :

GSTP1; GSTP1; PI; DFN7; GST3; GSTP; FAEES3; HEL-S-22; FAEES3; GST3

uniprot entry name :

GSTP1_HUMAN

reactivity :

Human

sequence length :

210

specificity :

No cross-reaction with other factors.

storage stability :

Store all reagents at 2-8 degree C.

other info1 :

Samples: Human serum, plasma or cell culture supernatant and organizations in the natural and recombinant GSTp concentration. Assay Type: Sandwich. Detection Range: 20 ng/ml-0.312 ng/ml. Sensitivity: 0.06 ng/ml.

other info2 :

Intra-assay Precision: <= 8%. Inter-assay Precision: <= 12%

products description :

Principle of the Assay This experiment use double-sandwich elisa technique and the ELISA Kit provided is typical. The pre-coated antibody is human GSTp monoclonal antibody and the detecting antibody is polyclonal antibody with biotin labeled. Samples and biotin labeling antibody are added into ELISA plate wells and washed out with PBS or TBS. Then Avidin-peroxidase conjugates are added to ELISA wells in order; Use TMB substrate for coloring after reactant thoroughly washed out by PBS or TBS. TMB turns into blue in peroxidase catalytic and finally turns into yellow under the action of acid. The color depth and the testing factors in samples are positively correlated.

ncbi gi num :

32187525

ncbi acc num :

AAP72967.1

ncbi mol weight :

23,356 Da

ncbi pathways :

4-hydroxy-2-nonenal Detoxification Pathway (703096); Arachidonate Epoxygenase / Epoxide Hydrolase Pathway (198838); Biological Oxidations Pathway (105698); Chemical Carcinogenesis Pathway (673221); Chemical Carcinogenesis Pathway (673237); Diurnally Regulated Genes With Circadian Orthologs Pathway (198813); Drug Metabolism - Cytochrome P450 Pathway (83032); Drug Metabolism - Cytochrome P450 Pathway (427); Glutathione Conjugation Pathway (105727); Glutathione Metabolism Pathway (82973)

ncbi summary :

Glutathione S-transferases (GSTs) are a family of enzymes that play an important role in detoxification by catalyzing the conjugation of many hydrophobic and electrophilic compounds with reduced glutathione. Based on their biochemical, immunologic, and structural properties, the soluble GSTs are categorized into 4 main classes: alpha, mu, pi, and theta. This GST family member is a polymorphic gene encoding active, functionally different GSTP1 variant proteins that are thought to function in xenobiotic metabolism and play a role in susceptibility to cancer, and other diseases. [provided by RefSeq, Jul 2008]

uniprot summary :

GSTP1: Conjugation of reduced glutathione to a wide number of exogenous and endogenous hydrophobic electrophiles. Regulates negatively CDK5 activity via p25/p35 translocation to prevent neurodegeneration. Homodimer. Interacts with CDK5. Belongs to the GST superfamily. Pi family. Protein type: Xenobiotic Metabolism - metabolism by cytochrome P450; Xenobiotic Metabolism - drug metabolism - cytochrome P450; EC 2.5.1.18; Other Amino Acids Metabolism - glutathione; Transferase. Chromosomal Location of Human Ortholog: 11q13. Cellular Component: extracellular space; mitochondrion; cytoplasm; plasma membrane; intracellular; cytosol; nucleus; vesicle. Molecular Function: JUN kinase binding; protein binding; glutathione transferase activity; kinase regulator activity. Biological Process: negative regulation of JNK activity; negative regulation of MAP kinase activity; central nervous system development; negative regulation of MAPKKK cascade; negative regulation of acute inflammatory response; negative regulation of interleukin-1 beta production; negative regulation of tumor necrosis factor production; negative regulation of I-kappaB kinase/NF-kappaB cascade; negative regulation of stress-activated MAPK cascade; regulation of stress-activated MAPK cascade; negative regulation of biosynthetic process; response to reactive oxygen species; glutathione metabolic process; negative regulation of fibroblast proliferation; negative regulation of nitric-oxide synthase biosynthetic process; positive regulation of superoxide release; xenobiotic metabolic process; negative regulation of protein kinase activity; negative regulation of apoptosis

size1 :

48-Strip-Wells

price1 :

275 USD

size2 :

96-Strip-Wells

price2 :

420

more info or order :

MyBioSource product webpage