Mouse Cyclic Guanosine Monophosphate (cGMP) ELISA Kit | MyBioSource MBS267678 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Mouse Cyclic Guanosine Monophosphate (cGMP) ELISA Kit

catalog :

MBS267678

quantity :

48-Strip-Wells

price :

290 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS267678

products type :

ELISA Kit

products full name :

Mouse Cyclic Guanosine Monophosphate (cGMP) ELISA Kit

products short name :

Cyclic Guanosine Monophosphate (cGMP)

other names :

cGMP-dependent protein kinase 1 isoform 1; cGMP-dependent protein kinase 1; cGMP-dependent protein kinase 1; protein kinase, cGMP-dependent, regulatory, type I, beta; protein kinase, cGMP-dependent, type I; cGMP-dependent protein kinase I; cGKI

products gene name :

cGMP

other gene names :

PRKG1; PRKG1; 1; PKG; cGK; AAT8; cGK1; cGKI; cGK 1; PRKG1B; PRKGR1B; cGKI-BETA; cGKI-alpha; PRKG1B; PRKGR1A; PRKGR1B; cGK 1; cGK1; cGKI

uniprot entry name :

KGP1_HUMAN

reactivity :

Mouse

sequence length :

671

specificity :

No cross-reaction with other factors.

storage stability :

Store all reagents at 2-8 degree C.

other info1 :

Samples: Mouse serum, plasma or cell culture supernatant and organizations in the natural and recombinant cGMP concentration. Assay Type: Quantitative Sandwich. Sensitivity: Up to 0.06 ng/ml.

other info2 :

Intra-assay Precision: <= 8%. Inter-assay Precision: <= 12%

products description :

Principle of the Assay: This experiment use double-sandwich elisa technique and the ELISA Kit provided is typical. The pre-coated antibody is Mouse cGMP monoclonal antibody and the detecting antibody is polyclonal antibody with biotin labeled. Samples and biotin labeling antibody are added into ELISA plate wells and washed out with PBS or TBS. Then Avidin-peroxidase conjugates are added to ELISA wells in order; Use TMB substrate for coloring after reactant thoroughly washed out by PBS or TBS. TMB turns into blue in peroxidase catalytic and finally turns into yellow under the action of acid. The color depth and the testing factors in samples are positively correlated.

ncbi gi num :

148612818

ncbi acc num :

NP_001091982.1

ncbi gb acc num :

NM_001098512.2

ncbi mol weight :

44,185 Da

ncbi pathways :

Adaptive Immune System Pathway (366160); Ca2+ Pathway (941399); Circadian Entrainment Pathway (698773); Circadian Entrainment Pathway (699872); Gap Junction Pathway (83072); Gap Junction Pathway (483); Hemostasis Pathway (106028); Immune System Pathway (106386); Long-term Depression Pathway (83086); Long-term Depression Pathway (497)

ncbi summary :

Mammals have three different isoforms of cyclic GMP-dependent protein kinase (Ialpha, Ibeta, and II). These PRKG isoforms act as key mediators of the nitric oxide/cGMP signaling pathway and are important components of many signal transduction processes in diverse cell types. This PRKG1 gene on human chromosome 10 encodes the soluble Ialpha and Ibeta isoforms of PRKG by alternative transcript splicing. A separate gene on human chromosome 4, PRKG2, encodes the membrane-bound PRKG isoform II. The PRKG1 proteins play a central role in regulating cardiovascular and neuronal functions in addition to relaxing smooth muscle tone, preventing platelet aggregation, and modulating cell growth. This gene is most strongly expressed in all types of smooth muscle, platelets, cerebellar Purkinje cells, hippocampal neurons, and the lateral amygdala. Isoforms Ialpha and Ibeta have identical cGMP-binding and catalytic domains but differ in their leucine/isoleucine zipper and autoinhibitory sequences and therefore differ in their dimerization substrates and kinase enzyme activity. [provided by RefSeq, Sep 2011]

uniprot summary :

PKG1: an AGC kinase of the PKG family. Type I cGMP-dependent protein kinase; relaxes vascular smooth muscle and inhibits platelet aggregation. Binding of cGMP results in enzyme activation. Two splice variant isoforms have been described. Protein type: Protein kinase, AGC; Kinase, protein; Protein kinase, Ser/Thr (non-receptor); EC 2.7.11.12; AGC group; PKG family. Chromosomal Location of Human Ortholog: 10q11.2. Cellular Component: Golgi apparatus; cytoplasm; plasma membrane; cytosol. Molecular Function: protein binding; calcium channel regulator activity; cGMP-dependent protein kinase activity; cGMP binding; ATP binding. Biological Process: regulation of GTPase activity; relaxation of vascular smooth muscle; cGMP-mediated signaling; forebrain development; neuron migration; dendrite development; signal transduction; blood coagulation; actin cytoskeleton organization and biogenesis; protein amino acid phosphorylation

size1 :

48-Strip-Wells

price1 :

290 USD

size2 :

96-Strip-Wells

price2 :

450

more info or order :

MyBioSource product webpage