ELISA/assay

Porcine Procollagen I C-Terminal Propeptide (PICP) ELISA KIT

MBS267455

48-Strip-Wells

315 USD

ELISA Kit

Porcine Procollagen I C-Terminal Propeptide (PICP) ELISA KIT

Procollagen I C-Terminal Propeptide (PICP)

procollagen C-endopeptidase enhancer 1; Procollagen C-endopeptidase enhancer 1; procollagen C-endopeptidase enhancer 1; procollagen C-proteinase enhancer 1; procollagen COOH-terminal proteinase enhancer 1; type 1 procollagen C-proteinase enhancer protein; type I procollagen COOH-terminal proteinase enhancer; procollagen, type 1, COOH-terminal proteinase enhancer; procollagen C-endopeptidase enhancer; Procollagen COOH-terminal proteinase enhancer 1; PCPE-1; Procollagen C-proteinase enhancer 1; Type 1 procollagen C-proteinase enhancer protein; Type I procollagen COOH-terminal proteinase enhancer

PICP

PCOLCE; PCOLCE; PCPE; PCPE1; PCPE-1; PCPE1; PCPE-1; Procollagen C-proteinase enhancer 1

PCOC1_HUMAN

Porcine

449

No cross-reaction with other factors.

Store all reagents at 2-8 degree C.

Samples: Porcine serum, plasma or Cell Culture Supernatant and organizations in the natural and recombinant PICP concentration. Assay Type: Sandwich. Detection Range: 50 ng/ml-0.78 ng/ml. Sensitivity: 0.08 ng/ml.

Intra-assay Precision: ? 8%. Inter-assay Precision: ? 12%

Principle of the assay: This experiment use double-sandwich elisa technique and the ELISA Kit provided is typical. The pre-coated antibody is Porcine PICP monoclonal antibody and the detecting antibody is polyclonal antibody with biotin labeled. Samples and biotin labeling antibody are added into ELISA plate wells and washed out with PBS or TBS. Then Avidin-peroxidase conjugates are added to ELISA wells in order; Use TMB substrate for coloring after reactant thoroughly washed out by PBS or TBS. TMB turns into blue in peroxidase catalytic and finally turns into yellow under the action of acid. The color depth and the testing factors in samples are positively correlated.

157653329

NP_002584.2

NM_002593.3

47,972 Da

Assembly Of Collagen Fibrils And Other Multimeric Structures Pathway (730306); Collagen Biosynthesis And Modifying Enzymes Pathway (645289); Collagen Formation Pathway (645288); Crosslinking Of Collagen Fibrils Pathway (730308); Extracellular Matrix Organization Pathway (576262)

Fibrillar collagen types I-III are synthesized as precursor molecules known as procollagens. These precursors contain amino- and carboxyl-terminal peptide extensions known as N- and C-propeptides, respectively, which are cleaved, upon secretion of procollagen from the cell, to yield the mature triple helical, highly structured fibrils. This gene encodes a glycoprotein which binds and drives the enzymatic cleavage of type I procollagen and heightens C-proteinase activity. [provided by RefSeq, Jul 2008]

PCOLCE: Binds to the C-terminal propeptide of type I procollagen and enhances procollagen C-proteinase activity. Protein type: Secreted, signal peptide; Secreted; Extracellular matrix. Chromosomal Location of Human Ortholog: 7q22. Cellular Component: extracellular matrix; extracellular space. Molecular Function: heparin binding; collagen binding; protein binding; protease activator activity. Biological Process: multicellular organismal development; proteolysis

48-Strip-Wells

315 USD

96-Strip-Wells

490