ELISA/assay

Mouse Glutathione (GSH) ELISA Kit

MBS267424

48-Strip-Wells

290 USD

ELISA Kit

Mouse Glutathione (GSH) ELISA Kit

Glutathione (GSH)

glutathione S-transferase Mu 2 isoform 2; Glutathione S-transferase Mu 2; glutathione S-transferase Mu 2; GST, muscle; GST class-mu 2; glutathione S-transferase 4; glutathione S-transferase M1; glutathione S-aryltransferase M2; glutathione S-alkyltransferase M2; glutathione S-aralkyltransferase M2; S-(hydroxyalkyl)glutathione lyase M2; glutathione S-transferase M2 (muscle); glutathione S-transferase mu 2 (muscle); GST class-mu 2; GSTM2-2

GSH

GSTM2; GSTM2; GST4; GSTM; GTHMUS; GSTM2-2; GST4

GSTM2_HUMAN

Mouse

191

No cross-reaction with other factors.

Store all reagents at 2-8 degree C.

Samples: Mouse serum, plasma or Cell Culture Supernatant and organizations in the natural and recombinant GSH concentration. Assay Type: Sandwich. Detection Range: 200 mug/ml-3.12 mug/ml. Sensitivity: 0.6 mug/ml.

Intra-assay Precision: <= 8%. Inter-assay Precision: <= 12%

Principle of the assay: This experiment use double-sandwich elisa technique and the ELISA Kit provided is typical. The pre-coated antibody is Mouse GSH monoclonal antibody and the detecting antibody is polyclonal antibody with biotin labeled. Samples and biotin labeling antibody are added into ELISA plate wells and washed out with PBS or TBS. Then Avidin-peroxidase conjugates are added to ELISA wells in order; Use TMB substrate for coloring after reactant thoroughly washed out by PBS or TBS. TMB turns into blue in peroxidase catalytic and finally turns into yellow under the action of acid. The color depth and the testing factors in samples are positively correlated.

215277000

NP_001135840.1

NM_001142368.1

22,644 Da

Biological Oxidations Pathway (105698); Chemical Carcinogenesis Pathway (673221); Chemical Carcinogenesis Pathway (673237); Drug Metabolism - Cytochrome P450 Pathway (83032); Drug Metabolism - Cytochrome P450 Pathway (427); Glutathione Conjugation Pathway (105727); Glutathione Metabolism Pathway (82973); Glutathione Metabolism Pathway (198824); Glutathione Metabolism Pathway (343); Metabolism Pathway (477135)

Cytosolic and membrane-bound forms of glutathione S-transferase are encoded by two distinct supergene families. At present, eight distinct classes of the soluble cytoplasmic mammalian glutathione S-transferases have been identified: alpha, kappa, mu, omega, pi, sigma, theta and zeta. This gene encodes a glutathione S-transferase that belongs to the mu class. The mu class of enzymes functions in the detoxification of electrophilic compounds, including carcinogens, therapeutic drugs, environmental toxins and products of oxidative stress, by conjugation with glutathione. The genes encoding the mu class of enzymes are organized in a gene cluster on chromosome 1p13.3 and are known to be highly polymorphic. These genetic variations can change an individual's susceptibility to carcinogens and toxins as well as affect the toxicity and efficacy of certain drugs. [provided by RefSeq, Jul 2008]

GSTM2: Conjugation of reduced glutathione to a wide number of exogenous and endogenous hydrophobic electrophiles. Belongs to the GST superfamily. Mu family. Protein type: Transferase; EC 2.5.1.18; Motility/polarity/chemotaxis. Chromosomal Location of Human Ortholog: 1p13.3. Cellular Component: sarcoplasmic reticulum; cytoplasm; cytosol. Molecular Function: enzyme binding; protein homodimerization activity; glutathione binding; glutathione transferase activity; receptor binding. Biological Process: regulation of skeletal muscle contraction via regulation of the release of sequestered calcium ion; glutathione metabolic process; nitrobenzene metabolic process; xenobiotic metabolic process; xenobiotic catabolic process

48-Strip-Wells

290 USD

96-Strip-Wells

450