Rat Orexin B ELISA Kit | MyBioSource MBS267360 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Rat Orexin B ELISA Kit

catalog :

MBS267360

quantity :

48-Strip-Wells

price :

290 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS267360

products type :

ELISA Kit

products full name :

Rat Orexin B ELISA Kit

products short name :

Orexin B

other names :

orexin; Orexin; orexin; prepro-orexin; hypocretin (orexin) neuropeptide precursor; Hypocretin; Hcrt

other gene names :

HCRT; HCRT; OX; PPOX; NRCLP1; OX; PPORX; PPOX; Hcrt; Hcrt1; Hcrt2

uniprot entry name :

OREX_HUMAN

reactivity :

Rat

sequence length :

131

storage stability :

Store all reagents at 2-8 degree C.

other info1 :

Samples: Rat serum, plasma or Cell Culture Supernatant and organizations in the natural and recombinant Orexin B concentration. Assay Type: Sandwich. Detection Range: 1000 pg/ml-15.6 pg/ml

products description :

Principle of the Assay: This experiment use double-sandwich elisa technique and the ELISA Kit provided is typical. The pre-coated antibody is Rat Orexin B monoclonal antibody and the detecting antibody is polyclonal antibody with biotin labeled. Samples and biotin labeling antibody are added into ELISA plate wells and washed out with PBS or TBS. Then Avidin-peroxidase conjugates are added to ELISA wells in order; Use TMB substrate for coloring after reactant thoroughly washed out by PBS or TBS. TMB turns into blue in peroxidase catalytic and finally turns into yellow under the action of acid. The color depth and the testing factors in samples are positively correlated.

ncbi gi num :

4557635

ncbi acc num :

NP_001515.1

ncbi gb acc num :

NM_001524.1

ncbi mol weight :

13,363 Da

ncbi pathways :

Class A/1 (Rhodopsin-like Receptors) Pathway (106357); G Alpha (q) Signalling Events Pathway (106043); GPCR Downstream Signaling Pathway (119548); GPCR Ligand Binding Pathway (161020); Gastrin-CREB Signalling Pathway Via PKC And MAPK (645295); Orexin And Neuropeptides FF And QRFP Bind To Their Respective Receptors Pathway (106362); Peptide Ligand-binding Receptors Pathway (106358); Signal Transduction Pathway (477114); Signaling By GPCR Pathway (106356)

ncbi summary :

This gene encodes a hypothalamic neuropeptide precursor protein that gives rise to two mature neuropeptides, orexin A and orexin B, by proteolytic processing. Orexin A and orexin B, which bind to orphan G-protein coupled receptors HCRTR1 and HCRTR2, function in the regulation of sleep and arousal. This neuropeptide arrangement may also play a role in feeding behavior, metabolism, and homeostasis. [provided by RefSeq, Jan 2010]

uniprot summary :

HCRT: Neuropeptides that play a significant role in the regulation of food intake and sleep-wakefulness, possibly by coordinating the complex behavioral and physiologic responses of these complementary homeostatic functions. A broader role in the homeostatic regulation of energy metabolism, autonomic function, hormonal balance and the regulation of body fluids, is also suggested. Orexin-A binds to both OX1R and OX2R with a high affinity, whereas orexin-B binds only to OX2R with a similar high affinity. Defects in HCRT are the cause of narcolepsy type 1 (NRCLP1). Narcolepsy is a neurological disabling sleep disorder, characterized by excessive daytime sleepiness, sleep fragmentation, symptoms of abnormal rapid-eye-movement (REM) sleep, such as cataplexy, hypnagogic hallucinations, and sleep paralysis. Cataplexy is a sudden loss of muscle tone triggered by emotions, which is the most valuable clinical feature used to diagnose narcolepsy. Human narcolepsy is primarily a sporadically occurring disorder but familial clustering has been observed. Human narcolepsy is associated with a deficient orexin system. Orexins are absent and/or greatly diminished in the brain and cerebrospinal fluid (CSF) of most narcoleptic patients. Belongs to the orexin family. Protein type: Hormone. Chromosomal Location of Human Ortholog: 17q21. Cellular Component: synaptic vesicle; rough endoplasmic reticulum; perinuclear region of cytoplasm; extracellular region; cell junction; secretory granule. Molecular Function: type 2 hypocretin receptor binding; type 1 hypocretin receptor binding. Biological Process: synaptic transmission; elevation of cytosolic calcium ion concentration; negative regulation of transmission of nerve impulse; neuropeptide signaling pathway; eating behavior; negative regulation of DNA replication; negative regulation of potassium ion transport; protein kinase C activation; regulation of neurotransmitter secretion; G-protein signaling, coupled to IP3 second messenger (phospholipase C activating); regulation of excitatory postsynaptic membrane potential; positive regulation of calcium ion transport; positive regulation of transmission of nerve impulse. Disease: Narcolepsy 1

size1 :

48-Strip-Wells

price1 :

290 USD

size2 :

96-Strip-Wells

price2 :

450

more info or order :

MyBioSource product webpage