ELISA/assay

Human growth-associated protein 43 (GAP-43) ELISA Kit

MBS267257

48-Strip-Wells

275 USD

ELISA Kit

Human growth-associated protein 43 (GAP-43) ELISA Kit

growth-associated protein 43 (GAP-43)

growth associated protein 43; Neuromodulin; neuromodulin; protein F1; neural phosphoprotein B-50; growth-associated protein 43; axonal membrane protein GAP-43; calmodulin-binding protein P-57; nerve growth-related peptide GAP43; neuron growth-associated protein 43; growth associated protein 43; Axonal membrane protein GAP-43; Growth-associated protein 43; Neural phosphoprotein B-50; pp46

GAP-43

GAP43; GAP43; B-50; PP46

NEUM_HUMAN

Human

238

No cross-reaction with other factors.

Store all reagents at 2-8 degree C.

Samples: Human serum, plasma or Cell Culture Supernatant and organizations in the natural and recombinant GAP-43 concentration. Assay Type: Sandwich. Detection Range: 10 ng/ml-0.156 ng/ml. Sensitivity: 0.05 ng/ml.

Intra-assay Precision: <= 8%. Inter-assay Precision: <= 12%

Principle of the Assay: This experiment use double-sandwich elisa technique and the ELISA Kit provided is typical. The pre-coated antibody is human GAP-43 monoclonal antibody and the detecting antibody is polyclonal antibody with biotin labeled. Samples and biotin labeling antibody are added into ELISA plate wells and washed out with PBS or TBS. Then Avidin-peroxidase conjugates are added to ELISA wells in order; Use TMB substrate for coloring after reactant thoroughly washed out by PBS or TBS. TMB turns into blue in peroxidase catalytic and finally turns into yellow under the action of acid. The color depth and the testing factors in samples are positively correlated.

119600000

EAW79594.1

28,766 Da

Glial Cell Differentiation Pathway (698758); N-cadherin Signaling Events Pathway (137919); Spinal Cord Injury Pathway (739007)

The protein encoded by this gene has been termed a 'growth' or 'plasticity' protein because it is expressed at high levels in neuronal growth cones during development and axonal regeneration. This protein is considered a crucial component of an effective regenerative response in the nervous system. Alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

GAP43: This protein is associated with nerve growth. It is a major component of the motile growth cones that form the tips of elongating axons. Plays a role in axonal and dendritic filopodia induction. Identified in a complex containing FGFR4, NCAM1, CDH2, PLCG1, FRS2, SRC, SHC1, GAP43 and CTTN. Binds calmodulin with a greater affinity in the absence of Ca(2+) than in its presence. Belongs to the neuromodulin family. 2 isoforms of the human protein are produced by alternative splicing. Protein type: Cell surface. Chromosomal Location of Human Ortholog: 3q13.31. Cellular Component: filopodium membrane; axon; cytoplasm; postsynaptic density; plasma membrane; cell junction. Molecular Function: calmodulin binding; protein binding. Biological Process: cell fate commitment; glial cell differentiation; tissue regeneration; regulation of growth; axon choice point recognition; protein kinase C activation; response to wounding; regulation of filopodium formation

48-Strip-Wells

275 USD

96-Strip-Wells

420