ELISA/assay

Human Vitamin D (VD) ELISA Kit

MBS267183

48-Strip-Wells

275 USD

ELISA Kit

Human Vitamin D (VD) ELISA Kit

Vitamin D (VD)

vitamin D 25-hydroxylase; Vitamin D 25-hydroxylase; vitamin D 25-hydroxylase; cytochrome P450 2R1; cytochrome P450, family 2, R1; cytochrome P450, family 2, subfamily R, polypeptide 1; Cytochrome P450 2R1

VD

CYP2R1; CYP2R1

CP2R1_HUMAN

Human

501

No cross-reaction with other factors.

Store all reagents at 2-8 degree C.

Samples: Human serum, plasma or Cell Culture Supernatant and organizations in the natural and recombinant VD concentration. Assay Type: Sandwich. Detection Range: 400 ng/ml-6.25 ng/ml. Sensitivity: 0.8 ng/ml.

Intra-assay Precision: <= 8%. Inter-assay Precision: <= 12%

Principle of the Assay: This experiment use double-sandwich elisa technique and the ELISA Kit provided is typical. The pre-coated antibody is human VD monoclonal antibody and the detecting antibody is polyclonal antibody with biotin labeled. Samples and biotin labeling antibody are added into ELISA plate wells and washed out with PBS or TBS. Then Avidin-peroxidase conjugates are added to ELISA wells in order; Use TMB substrate for coloring after reactant thoroughly washed out by PBS or TBS. TMB turns into blue in peroxidase catalytic and finally turns into yellow under the action of acid. The color depth and the testing factors in samples are positively correlated.

45267826

NP_078790.2

NM_024514.4

57,359 Da

1,25-dihydroxyvitamin D3 Biosynthesis Pathway (138457); Biological Oxidations Pathway (105698); Cholecalciferol Biosynthesis Pathway (413391); Cholecalciferol Biosynthesis Pathway (468294); Cytochrome P450 - Arranged By Substrate Type Pathway (105700); Metabolic Pathways (132956); Metabolism Pathway (477135); Metabolism Of Lipids And Lipoproteins Pathway (160976); Metabolism Of Steroid Hormones And Vitamin D Pathway (106150); Phase 1 - Functionalization Of Compounds Pathway (105699)

This gene encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This enzyme is a microsomal vitamin D hydroxylase that converts vitamin D into the active ligand for the vitamin D receptor. A mutation in this gene has been associated with selective 25-hydroxyvitamin D deficiency. [provided by RefSeq, Jul 2008]

CYP2R1: Has a D-25-hydroxylase activity on both forms of vitamin D, vitamin D(2) and D(3). Defects in CYP2R1 are the cause of rickets vitamin D- dependent type 1B (VDDR1B); also known as pseudovitamin D(3) deficiency rickets due to 25-hydroxylase deficiency. A disorder caused by a selective deficiency of the active form of vitamin D (1,25-dihydroxyvitamin D3) and resulting in defective bone mineralization and clinical features of rickets. The patients sera have low calcium concentrations, low phosphate concentrations, elevated alkaline phosphatase activityand low levels of 25-hydroxyvitamin D. Belongs to the cytochrome P450 family. Protein type: Oxidoreductase; EC 1.14.13.15. Chromosomal Location of Human Ortholog: 11p15.2. Cellular Component: endoplasmic reticulum membrane; intracellular membrane-bound organelle; cytoplasm. Molecular Function: iron ion binding; heme binding; oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, reduced flavin or flavoprotein as one donor, and incorporation of one atom of oxygen; steroid hydroxylase activity; oxygen binding; vitamin D3 25-hydroxylase activity. Biological Process: organic acid metabolic process; steroid metabolic process; vitamin metabolic process; xenobiotic metabolic process; exogenous drug catabolic process; vitamin D metabolic process. Disease: Vitamin D Hydroxylation-deficient Rickets, Type 1b

48-Strip-Wells

275 USD

96-Strip-Wells

420