Human Tissue Inhibitors Of Metalloproteinase 1 (TIMP-1) ELISA Kit | MyBioSource MBS267108 product information

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product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Human Tissue Inhibitors Of Metalloproteinase 1 (TIMP-1) ELISA Kit

catalog :

MBS267108

quantity :

48-Strip-Wells

price :

250 USD

product information

catalog number :

MBS267108

products type :

ELISA Kit

products full name :

Human Tissue Inhibitors Of Metalloproteinase 1 (TIMP-1) ELISA Kit

products short name :

[Tissue Inhibitors Of Metalloproteinase 1 (TIMP-1)]

other names :

[tissue inhibitor of metalloproteinase 1, partial; Metalloproteinase inhibitor 1; metalloproteinase inhibitor 1; TIMP-1; collagenase inhibitor; erythroid potentiating activity; erythroid-potentiating activity; fibroblast collagenase inhibitor; tissue inhibitor of metalloproteinases 1; TIMP metallopeptidase inhibitor 1; Erythroid-potentiating activity; EPA; Fibroblast collagenase inhibitor; Collagenase inhibitor; Tissue inhibitor of metalloproteinases 1; TIMP-1]

products gene name :

[TIMP-1]

other gene names :

[TIMP1; TIMP1; EPA; EPO; HCI; CLGI; TIMP; CLGI; TIMP; EPA; Collagenase inhibitor; TIMP-1]

uniprot entry name :

TIMP1_HUMAN

reactivity :

Human

sequence length :

specificity :

No cross-reaction with other factors.

storage stability :

Store all reagents at 2-8 degree C.

image1 heading :

Typical Testing Data/Standard Curve (for reference only)

other info1 :

Samples: Serum, plasma or cell culture supernatant and organizations in the natural and recombinant TIMP-1 concentration. Assay Type: Sandwich. Detection Range: 10 ng/ml-0.156 ng/ml. Sensitivity: 0.05 ng/ml.

other info2 :

Intra-assay Precision: <= 8%. Inter-assay Precision: <= 12%

products description :

Principle of the Assay: This experiment use double-sandwich elisa technique and the ELISA Kit provided is typical. The pre-coated antibody is human TIMP-1 monoclonal antibody and the detecting antibody is polyclonal antibody with biotin labeled. Samples and biotin labeling antibody are added into ELISA plate wells and washed out with PBS or TBS. Then Avidin-peroxidase conjugates are added to ELISA wells in order; Use TMB substrate for coloring after reactant thoroughly washed out by PBS or TBS. TMB turns into blue in peroxidase catalytic and finally turns into yellow under the action of acid. The color depth and the testing factors in samples are positively correlated.

ncbi gi num :

994731

ncbi acc num :

AAA75558.1

ncbi mol weight :

23,171 Da

ncbi pathways :

Activation Of Matrix Metalloproteinases Pathway (576264); Degradation Of The Extracellular Matrix Pathway (576263); Extracellular Matrix Organization Pathway (576262); HIF-1 Signaling Pathway (695200); Hemostasis Pathway (106028); IL6-mediated Signaling Events Pathway (137932); Matrix Metalloproteinases Pathway (198900); Platelet Activation, Signaling And Aggregation Pathway (106034); Platelet Degranulation Pathway (106050); Response To Elevated Platelet Cytosolic Ca2+ Pathway (106048)

ncbi summary :

This gene belongs to the TIMP gene family. The proteins encoded by this gene family are natural inhibitors of the matrix metalloproteinases (MMPs), a group of peptidases involved in degradation of the extracellular matrix. In addition to its inhibitory role against most of the known MMPs, the encoded protein is able to promote cell proliferation in a wide range of cell types, and may also have an anti-apoptotic function. Transcription of this gene is highly inducible in response to many cytokines and hormones. In addition, the expression from some but not all inactive X chromosomes suggests that this gene inactivation is polymorphic in human females. This gene is located within intron 6 of the synapsin I gene and is transcribed in the opposite direction. [provided by RefSeq, Jul 2008]

uniprot summary :

TIMP1: Complexes with metalloproteinases (such as collagenases) and irreversibly inactivates them by binding to their catalytic zinc cofactor. Also mediates erythropoiesis in vitro; but, unlike IL-3, it is species-specific, stimulating the growth and differentiation of only human and murine erythroid progenitors. Known to act on MMP-1, MMP-2, MMP-3, MMP-7, MMP-8, MMP-9, MMP-10, MMP-11, MMP-12, MMP-13 and MMP-16. Does not act on MMP-14. Belongs to the protease inhibitor I35 (TIMP) family. Protein type: Secreted; Secreted, signal peptide; Motility/polarity/chemotaxis. Chromosomal Location of Human Ortholog: Xp11.3-p11.23. Cellular Component: proteinaceous extracellular matrix; extracellular space; extracellular region; basement membrane. Molecular Function: metalloendopeptidase inhibitor activity; protein binding; growth factor activity; protease binding; metal ion binding; cytokine activity. Biological Process: platelet activation; response to peptide hormone stimulus; extracellular matrix disassembly; negative regulation of metalloenzyme activity; extracellular matrix organization and biogenesis; platelet degranulation; response to cytokine stimulus; positive regulation of cell proliferation; response to hormone stimulus; negative regulation of membrane protein ectodomain proteolysis; blood coagulation; negative regulation of apoptosis; aging

size1 :

48-Strip-Wells

price1 :

250 USD

size2 :

96-Strip-Wells

price2 :

365

size3 :

5x96-Strip-Wells

price3 :

1480

size4 :

10x96-Strip-Wells

price4 :

2620