Human leukocyte antigen G (HLA-G) ELISA Kit | MyBioSource MBS267094 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Human leukocyte antigen G (HLA-G) ELISA Kit

catalog :

MBS267094

quantity :

48-Strip-Wells

price :

275 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS267094

products type :

ELISA Kit

products full name :

Human leukocyte antigen G (HLA-G) ELISA Kit

products short name :

leukocyte antigen G (HLA-G)

other names :

leukocyte antigen, HLA-A2 variant, partial; Leukocyte antigen, HLA-A2 variant; HLA class I histocompatibility antigen, A-1 alpha chain; antigen presenting molecule; leukocyte antigen class I-A; MHC class I antigen HLA-A heavy chain; major histocompatibility complex, class I, A; Leukocyte antigen, HLA-A2 variant

products gene name :

HLA-G

other gene names :

HLA-A; HLAA

uniprot entry name :

O02922_HUMAN

reactivity :

Human

sequence length :

256

specificity :

No cross-reaction with other factors.

storage stability :

Store all reagents at 2-8 degree C.

other info1 :

Samples: Human serum, plasma or Cell Culture Supernatant and organizations in the natural and recombinant HLA-G concentration. Assay Type: Sandwich. Detection Range: 200 U/ml-3.12 U/ml. Sensitivity: 0.6U/ml.

other info2 :

Intra-assay Precision: <= 8%. Inter-assay Precision: <= 12%

products description :

. Principle of the assay: This experiment use double-sandwich elisa technique and the ELISA Kit provided is typical. The pre-coated antibody is human HLA-G monoclonal antibody and the detecting antibody is polyclonal antibody with biotin labeled. Samples and biotin labeling antibody are added into ELISA plate wells and washed out with PBS or TBS. Then Avidin-peroxidase conjugates are added to ELISA wells in order; Use TMB substrate for coloring after reactant thoroughly washed out by PBS or TBS. TMB turns into blue in peroxidase catalytic and finally turns into yellow under the action of acid. The color depth and the testing factors in samples are positively correlated.

ncbi gi num :

2117163

ncbi acc num :

CAA73716.1

uniprot acc num :

O02922

ncbi mol weight :

29,434 Da

ncbi pathways :

Adaptive Immune System Pathway (366160); Allograft Rejection Pathway (920963); Allograft Rejection Pathway (83123); Allograft Rejection Pathway (535); Antigen Presentation: Folding, Assembly And Peptide Loading Of Class I MHC Pathway (366163); Antigen Processing And Presentation Pathway (83074); Antigen Processing And Presentation Pathway (485); Antigen Processing-Cross Presentation Pathway (477122); Autoimmune Thyroid Disease Pathway (83121); Autoimmune Thyroid Disease Pathway (533)

ncbi summary :

HLA-A belongs to the HLA class I heavy chain paralogues. This class I molecule is a heterodimer consisting of a heavy chain and a light chain (beta-2 microglobulin). The heavy chain is anchored in the membrane. Class I molecules play a central role in the immune system by presenting peptides derived from the endoplasmic reticulum lumen. They are expressed in nearly all cells. The heavy chain is approximately 45 kDa and its gene contains 8 exons. Exon 1 encodes the leader peptide, exons 2 and 3 encode the alpha1 and alpha2 domains, which both bind the peptide, exon 4 encodes the alpha3 domain, exon 5 encodes the transmembrane region, and exons 6 and 7 encode the cytoplasmic tail. Polymorphisms within exon 2 and exon 3 are responsible for the peptide binding specificity of each class one molecule. Typing for these polymorphisms is routinely done for bone marrow and kidney transplantation. Hundreds of HLA-A alleles have been described. [provided by RefSeq, Jul 2008]

uniprot summary :

HLAA: Involved in the presentation of foreign antigens to the immune system. Belongs to the MHC class I family. Protein type: Immunoglobulin superfamily; Membrane protein, integral. Chromosomal Location of Human Ortholog: 6p21.3. Cellular Component: Golgi membrane; Golgi apparatus; phagocytic vesicle membrane; cell surface; membrane; integral to plasma membrane; early endosome membrane; endoplasmic reticulum; plasma membrane; MHC class I protein complex; Golgi medial cisterna. Molecular Function: protein binding; peptide antigen binding; beta-2-microglobulin binding; T cell receptor binding; TAP binding; receptor binding. Biological Process: regulation of immune response; viral reproduction; detection of bacterium; positive regulation of T cell mediated cytotoxicity; antigen processing and presentation of exogenous peptide antigen via MHC class I, TAP-independent; cytokine and chemokine mediated signaling pathway; antigen processing and presentation of exogenous peptide antigen via MHC class I, TAP-dependent; protection from natural killer cell mediated cytotoxicity; regulation of defense response to virus by virus; positive regulation of interferon-gamma production; antigen processing and presentation of peptide antigen via MHC class I; antigen processing and presentation of endogenous peptide antigen via MHC class I via ER pathway, TAP-independent; antigen processing and presentation of exogenous peptide antigen via MHC class I; immune response. Disease: Severe Cutaneous Adverse Reaction, Susceptibility To

size1 :

48-Strip-Wells

price1 :

275 USD

size2 :

96-Strip-Wells

price2 :

420

more info or order :

MyBioSource product webpage