catalog number :
MBS267071
products type :
ELISA Kit
products full name :
Chicken Vitamin B12 (VB12) ELISA Kit
products short name :
Vitamin B12 (VB12)
other names :
vitamin-B12 independent methionine synthase; Methionine synthase vitamin-B12 independent; vitamin-B12 independent methionine synthase; Methionine synthase vitamin-B12 independent
products gene name :
VB12
other gene names :
Halhy_4313
uniprot entry name :
F4KPQ7_HALH1
specificity :
No cross-reaction with other factors.
storage stability :
Store all reagents at 2-8 degree C.
other info1 :
Samples: Chicken serum, plasma or Cell Culture Supernatant and organizations in the natural and recombinant VB12 concentration. Assay Type: Sandwich. Detection Range: 2000 pg/ml-31.2 pg/ml. Sensitivity: the minimum detectable Chicken VB12 up to 12 pg/ml.
other info2 :
Intra-assay Precision: <= 8%. Inter-assay Precision: <= 12%
products description :
Principle of the assay: This experiment use double-sandwich elisa technique and the ELISA Kit provided is typical. The pre-coated antibody is Chicken VB12 monoclonal antibody and the detecting antibody is polyclonal antibody with biotin labeled. Samples and biotin labeling antibody are added into ELISA plate wells and washed out with PBS or TBS. Then Avidin-peroxidase conjugates are added to ELISA wells in order; Use TMB substrate for coloring after reactant thoroughly washed out by PBS or TBS. TMB turns into blue in peroxidase catalytic and finally turns into yellow under the action of acid. The color depth and the testing factors in samples are positively correlated.
ncbi acc num :
YP_004449030.1
ncbi gb acc num :
NC_015510.1
ncbi mol weight :
38,688 Da
ncbi pathways :
Biosynthesis Of Amino Acids Pathway (790698); Biosynthesis Of Amino Acids Pathway (795174); Biosynthesis Of Secondary Metabolites Pathway (217456); Cysteine And Methionine Metabolism Pathway (217387); Cysteine And Methionine Metabolism Pathway (103421); Metabolic Pathways (217448); Selenocompound Metabolism Pathway (217404); Selenocompound Metabolism Pathway (338)