ELISA/assay

Human Prostacyclin (PGI2) ELISA Kit

MBS267026

48-Strip-Wells

275 USD

ELISA Kit

Human Prostacyclin (PGI2) ELISA Kit

Prostacyclin (PGI2)

prostacyclin receptor; Prostacyclin receptor; prostacyclin receptor; PGI receptor; PGI2 receptor; prostanoid IP receptor; prostaglandin I2 receptor; prostaglandin I2 (prostacyclin) receptor (IP); Prostaglandin I2 receptor; PGI receptor; PGI2 receptor; Prostanoid IP receptor

PGI2

PTGIR; PTGIR; IP; PRIPR; PRIPR; PGI receptor; PGI2 receptor

PI2R_HUMAN

Human

386

No cross-reaction with other factors.

Store all reagents at 2-8 degree C.

Samples: Human serum, plasma or Cell Culture Supernatant and organizations in the natural and recombinant PGI2 concentration. Assay Type: Sandwich. Detection Range: 2000 pg/ml-31.2 pg/ml. Sensitivity: 12 pg/ml.

Intra-assay Precision: ? 8%. Inter-assay Precision: ? 12%

Principle of the assay: This experiment use double-sandwich elisa technique and the ELISA Kit provided is typical. The pre-coated antibody is human PGI2 monoclonal antibody and the detecting antibody is polyclonal antibody with biotin labeled. Samples and biotin labeling antibody are added into ELISA plate wells and washed out with PBS or TBS. Then Avidin-peroxidase conjugates are added to ELISA wells in order; Use TMB substrate for coloring after reactant thoroughly washed out by PBS or TBS. TMB turns into blue in peroxidase catalytic and finally turns into yellow under the action of acid. The color depth and the testing factors in samples are positively correlated.

4506263

NP_000951.1

NM_000960.3

40,956 Da

Class A/1 (Rhodopsin-like Receptors) Pathway (106357); Eicosanoid Ligand-binding Receptors Pathway (106370); Endothelin Pathway (672453); G Alpha (s) Signalling Events Pathway (119549); GPCR Downstream Signaling Pathway (119548); GPCR Ligand Binding Pathway (161020); GPCRs, Class A Rhodopsin-like Pathway (198886); Hemostasis Pathway (106028); Neuroactive Ligand-receptor Interaction Pathway (83053); Neuroactive Ligand-receptor Interaction Pathway (462)

The protein encoded by this gene is a member of the G-protein coupled receptor family 1 and has been shown to be a receptor for prostacyclin. Prostacyclin, the major product of cyclooxygenase in macrovascular endothelium, elicits a potent vasodilation and inhibition of platelet aggregation through binding to this receptor. [provided by RefSeq, Jul 2008]

PTGIR: Receptor for prostacyclin (prostaglandin I2 or PGI2). The activity of this receptor is mediated by G(s) proteins which activate adenylate cyclase. Belongs to the G-protein coupled receptor 1 family. Protein type: GPCR, family 1; Membrane protein, integral; Membrane protein, multi-pass; Receptor, GPCR. Chromosomal Location of Human Ortholog: 19q13.3. Cellular Component: integral to plasma membrane; plasma membrane; cytosol. Molecular Function: G-protein coupled receptor activity; guanyl-nucleotide exchange factor activity. Biological Process: G-protein signaling, coupled to cyclic nucleotide second messenger; cell-cell signaling; negative regulation of smooth muscle cell proliferation; positive regulation of cAMP biosynthetic process; response to lipopolysaccharide; blood coagulation; G-protein signaling, adenylate cyclase activating pathway; positive regulation of GTPase activity

48-Strip-Wells

275 USD

96-Strip-Wells

420