ELISA/assay

Rat Pyridinoline (PYD) ELISA Kit

MBS266804

48-Strip-Wells

290 USD

ELISA Kit

Rat Pyridinoline (PYD) ELISA Kit

Pyridinoline (PYD)

Peptidyl-prolyl cis-trans isomerase FKBP14; Peptidyl-prolyl cis-trans isomerase FKBP14; peptidyl-prolyl cis-trans isomerase FKBP14; FKBP-22; rotamase; 22 kDa FKBP; PPIase FKBP14; FK506-binding protein 14; 22 kDa FK506-binding protein; FK506 binding protein 14, 22 kDa; 22 kDa FK506-binding protein; 22 kDa FKBP; FKBP-22; FK506-binding protein 14; FKBP-14; Rotamase

PYD

FKBP14; FKBP14; EDSKMH; FKBP22; IPBP12; FKBP22; UNQ322/PRO381; PPIase FKBP14; 22 kDa FKBP; FKBP-22; FKBP-14

FKB14_HUMAN

Rat

211

No cross-reaction with other factors.

Store all reagents at 2-8 degree C.

Samples: Rat serum, plasma or Cell Culture Supernatant and organizations in the natural and recombinant PYD concentration. Assay Type: Sandwich. Detection Range: 400 nmol/L-6.25 nmol/L. Sensitivity: 1 nmol/L.

Intra-assay Precision: ? 8%. Inter-assay Precision: ? 12%

Principle of the assay: This experiment use double-sandwich elisa technique and the ELISA Kit provided is typical. The pre-coated antibody is Rat PYD monoclonal antibody and the detecting antibody is polyclonal antibody with biotin labeled. Samples and biotin labeling antibody are added into ELISA plate wells and washed out with PBS or TBS. Then Avidin-peroxidase conjugates are added to ELISA wells in order; Use TMB substrate for coloring after reactant thoroughly washed out by PBS or TBS. TMB turns into blue in peroxidase catalytic and finally turns into yellow under the action of acid. The color depth and the testing factors in samples are positively correlated.

23821568

Q9NWM8.1

24,172 Da

IRE1alpha Activates Chaperones Pathway (105906); Metabolism Of Proteins Pathway (106230); Unfolded Protein Response (UPR) Pathway (105904); XBP1(S) Activates Chaperone Genes Pathway (530771)

The protein encoded by this gene is a member of the FK506-binding protein family of peptidyl-prolyl cis-trans isomerases. The encoded protein is found in the lumen of the endoplasmic reticulum, where it is thought to accelerate protein folding. Defects in this gene are a cause of a type of Ehlers-Danlos syndrome (EDS). Both a protein-coding variant and noncoding variants are transcribed from this gene. [provided by RefSeq, Mar 2012]

FKBP14: PPIases accelerate the folding of proteins during protein synthesis. Defects in FKBP14 are the cause of Ehlers-Danlos syndrome with progressive kyphoscoliosis, myopathy, and hearing loss (EDSKMH). A syndrome with features of Ehlers-Danlos syndrome types VIA and VIB on the one hand, and the collagen VI- related congenital myopathies Ullrich congenital muscular dystrophy and Bethlem myopathy on the other hand. Clinically, this disorder is characterized by the following features: severe generalized hypotonia at birth with marked muscle weakness that improve in infancy; early-onset progressive kyphoscoliosis; joint hypermobility without contractures; hyperelastic skin with follicular hyperkeratosis, easy bruising, and occasional abnormal scarring; myopathy as confirmed by muscle MRI, histology, and electron microscopy; hearing impairment, which is predominantly sensorineural; and a normal ratio of lysyl pyridinoline to hydroxylysyl pyridinoline (LP/HP) in urine. Protein type: Isomerase; EC 5.2.1.8; Secreted; Secreted, signal peptide. Chromosomal Location of Human Ortholog: 7p14.3. Cellular Component: endoplasmic reticulum membrane; endoplasmic reticulum lumen. Molecular Function: FK506 binding; peptidyl-prolyl cis-trans isomerase activity; calcium ion binding. Biological Process: cellular protein metabolic process; protein peptidyl-prolyl isomerization; unfolded protein response, activation of signaling protein activity; unfolded protein response. Disease: Ehlers-danlos Syndrome With Progressive Kyphoscoliosis, Myopathy, And Hearing Loss

48-Strip-Wells

290 USD

96-Strip-Wells

450