ELISA/assay

Human Surfactant Associated Protein D (SPD)

MBS266801

48-Strip-Wells

275 USD

ELISA Kit

Human Surfactant Associated Protein D (SPD)

Surfactant Associated Protein D (SPD)

Surfactant associated protein D; Pulmonary surfactant-associated protein D; pulmonary surfactant-associated protein D; PSP-D; lung surfactant protein D; surfactant associated protein D; Lung surfactant protein D

SPD

Sftpd; Sftpd; SP-D; Sftp4; AI573415; Sftp4; PSP-D; SP-D

SFTPD_MOUSE

Human

374

No cross-reaction with other factors.

Store all reagents at 2-8 degree C.

Samples: Human serum, plasma or Cell Culture Supernatant and organizations in the natural and recombinant SPD concentration. Assay Type: Sandwich. Detection Range: 200 ng/ml-3.12 ng/ml. Sensitivity: 0.6 ng/ml.

Intra-assay Precision: <= 8%. Inter-assay Precision: <= 12%

Principle of the assay: This experiment use double-sandwich elisa technique and the ELISA Kit provided is typical. The pre-coated antibody is human SPD monoclonal antibody and the detecting antibody is polyclonal antibody with biotin labeled. Samples and biotin labeling antibody are added into ELISA plate wells and washed out with PBS or TBS. Then Avidin-peroxidase conjugates are added to ELISA wells in order; Use TMB substrate for coloring after reactant thoroughly washed out by PBS or TBS. TMB turns into blue in peroxidase catalytic and finally turns into yellow under the action of acid. The color depth and the testing factors in samples are positively correlated.

13277590

AAH03705.1

37,688 Da

Phagosome Pathway (153914); Phagosome Pathway (153859)

SFTPD: Contributes to the lung s defense against inhaled microorganisms. May participate in the extracellular reorganization or turnover of pulmonary surfactant. Binds strongly maltose residues and to a lesser extent other alpha-glucosyl moieties. Belongs to the SFTPD family. Protein type: Secreted, signal peptide; Secreted. Cellular Component: multivesicular body; extracellular space; proteinaceous extracellular matrix; collagen; rough endoplasmic reticulum; cytoplasm; extracellular region; cytoplasmic vesicle. Molecular Function: monosaccharide binding; identical protein binding; lipopolysaccharide binding; carbohydrate binding. Biological Process: negative regulation of phagocytosis; negative regulation of T cell proliferation; immune system process; positive regulation of phagocytosis; innate immune response; respiratory gaseous exchange; opsonization; regulation of liquid surface tension; surfactant homeostasis; alveolus development; negative regulation of interleukin-2 production

48-Strip-Wells

275 USD

96-Strip-Wells

420