Human Phospholipase C (PLC) ELISA Kit | MyBioSource MBS266687 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Human Phospholipase C (PLC) ELISA Kit

catalog :

MBS266687

quantity :

48-Strip-Wells

price :

275 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS266687

products type :

ELISA Kit

products full name :

Human Phospholipase C (PLC) ELISA Kit

products short name :

Phospholipase C (PLC)

other names :

phospholipase C; 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase gamma-2; 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase gamma-2; phospholipase C-IV; phosphoinositide phospholipase C-gamma-2; phospholipase C, gamma 2 (phosphatidylinositol-specific); Phosphoinositide phospholipase C-gamma-2; Phospholipase C-IV; PLC-IV; Phospholipase C-gamma-2; PLC-gamma-2

products gene name :

PLC

other gene names :

PLCG2; PLCG2; FCAS3; APLAID; PLC-IV; PLC-gamma-2; PLC-IV; PLC-gamma-2

uniprot entry name :

PLCG2_HUMAN

reactivity :

Human

sequence length :

1252

specificity :

No cross-reaction with other factors.

storage stability :

Store all reagents at 2-8 degree C.

other info1 :

Samples: Human serum, plasma or Cell Culture Supernatant and organizations in the natural and recombinant PLC concentration. Assay Type: Sandwich. Detection Range: 10 ng/ml-0.156 ng/ml. Sensitivity: 0.05 ng/ml.

other info2 :

Intra-assay Precision: ? 8%. Inter-assay Precision: ? 12%

products description :

Princple of the assay: This experiment use double-sandwich elisa technique and the ELISA Kit provided is typical. The pre-coated antibody is human PLC monoclonal antibody and the detecting antibody is polyclonal antibody with biotin labeled. Samples and biotin labeling antibody are added into ELISA plate wells and washed out with PBS or TBS. Then Avidin-peroxidase conjugates are added to ELISA wells in order; Use TMB substrate for coloring after reactant thoroughly washed out by PBS or TBS. TMB turns into blue in peroxidase catalytic and finally turns into yellow under the action of acid. The color depth and the testing factors in samples are positively correlated.

ncbi gi num :

190036

ncbi acc num :

AAA60112.1

ncbi mol weight :

147,870 Da

ncbi pathways :

Adaptive Immune System Pathway (366160); Antigen Activates B Cell Receptor Leading To Generation Of Second Messengers Pathway (576249); B Cell Receptor Signaling Pathway (198909); B Cell Receptor Signaling Pathway (83081); B Cell Receptor Signaling Pathway (492); BCR Signaling Pathway (138058); Calcium Signaling Pathway (83050); Calcium Signaling Pathway (459); Class I PI3K Signaling Events Pathway (138022); D-myo-inositol (1,4,5)-trisphosphate Biosynthesis Pathway (545326)

ncbi summary :

The protein encoded by this gene is a transmembrane signaling enzyme that catalyzes the conversion of 1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate to 1D-myo-inositol 1,4,5-trisphosphate (IP3) and diacylglycerol (DAG) using calcium as a cofactor. IP3 and DAG are second messenger molecules important for transmitting signals from growth factor receptors and immune system receptors across the cell membrane. Mutations in this gene have been found in autoinflammation, antibody deficiency, and immune dysregulation syndrome and familial cold autoinflammatory syndrome 3. [provided by RefSeq, Mar 2014]

uniprot summary :

PLCG2: a calcium dependent phosphatidylinositol-specific phospholipase C. The activated enzyme produces the second messenger molecules diacylglycerol and inositol 1,4,5-trisphosphate. It is a crucial enzyme in transmembrane signaling. Phosphorylated and activated by tyrosine kinases in response to signaling through a variety of growth factor receptors and immune system receptors. Protein type: EC 3.1.4.11; Motility/polarity/chemotaxis; Phospholipase; Carbohydrate Metabolism - inositol phosphate. Chromosomal Location of Human Ortholog: 16q24.1. Cellular Component: plasma membrane; cytosol. Molecular Function: signal transducer activity; protein binding; phosphoinositide phospholipase C activity; phospholipase C activity. Biological Process: platelet activation; inositol phosphate metabolic process; Wnt receptor signaling pathway; phospholipid catabolic process; calcium-mediated signaling; response to lipopolysaccharide; T cell receptor signaling pathway; B cell receptor signaling pathway; activation of store-operated calcium channel activity; regulation of gene expression; inositol trisphosphate biosynthetic process; release of sequestered calcium ion into cytosol; B cell differentiation; negative regulation of programmed cell death; phosphatidylinositol biosynthetic process; innate immune response; follicular B cell differentiation; blood coagulation. Disease: Autoinflammation, Antibody Deficiency, And Immune Dysregulation, Plcg2-associated; Familial Cold Autoinflammatory Syndrome 3

size1 :

48-Strip-Wells

price1 :

275 USD

size2 :

96-Strip-Wells

price2 :

420

more info or order :

MyBioSource product webpage