catalog number :
MBS266685
products type :
ELISA Kit
products full name :
Human Complement 3 (C3) ELISA Kit
products short name :
Complement 3 (C3)
other names :
C3 and PZP-like alpha-2-macroglobulin domain-containing protein 8; C3 and PZP-like alpha-2-macroglobulin domain-containing protein 8; C3 and PZP-like alpha-2-macroglobulin domain-containing protein 8; alpha-2 macroglobulin family protein VIP; C3 and PZP-like, alpha-2-macroglobulin domain containing 8
other gene names :
CPAMD8; CPAMD8; VIP; K-CAP; KIAA1283
uniprot entry name :
CPMD8_HUMAN
specificity :
No cross-reaction with other factors.
storage stability :
Store all reagents at 2-8 degree C.
other info1 :
Samples: Human serum, plasma or Cell Culture Supernatant and organizations in the natural and recombinant C3 concentration. Assay Type: Sandwich. Detection Range: 300 ?g/ml-4.7 ?g/ml. Sensitivity: 0.7 ?g/ml.
other info2 :
Intra-assay Precision: ? 8%. Inter-assay Precision: ? 12%
products description :
Principle of the assay: This experiment use double-sandwich elisa technique and the ELISA Kit provided is typical. The pre-coated antibody is human C3 monoclonal antibody and the detecting antibody is polyclonal antibody with biotin labeled. Samples and biotin labeling antibody are added into ELISA plate wells and washed out with PBS or TBS. Then Avidin-peroxidase conjugates are added to ELISA wells in order; Use TMB substrate for coloring after reactant thoroughly washed out by PBS or TBS. TMB turns into blue in peroxidase catalytic and finally turns into yellow under the action of acid. The color depth and the testing factors in samples are positively correlated.
ncbi acc num :
NP_056507.2
ncbi gb acc num :
NM_015692.2
ncbi mol weight :
199,171 Da
ncbi summary :
CPAMD8 belongs to the complement component-3 (C3; MIM 120700)/alpha-2-macroglobulin (A2M; MIM 103950) family of proteins, which are involved in innate immunity and damage control. Complement components recognize and eliminate pathogens by direct binding or by mediating opsonization/phagocytosis and intracellular killing, and A2M is a broad-spectrum protease inhibitor (Li et al., 2004 [PubMed 15177561]).[supplied by OMIM, Mar 2008]
uniprot summary :
CPAMD8: Belongs to the protease inhibitor I39 (alpha-2- macroglobulin) family. Up-regulated by IL1B/interleukin-1 beta and IL6/interleukin-6. Interacts with heparin. 2 isoforms of the human protein are produced by alternative splicing. Chromosomal Location of Human Ortholog: 19p13.11. Cellular Component: extracellular space; plasma membrane. Molecular Function: serine-type endopeptidase inhibitor activity