ELISA/assay

Human growth hormone-binding protein (GHBP) ELISA Kit

MBS266601

48-Strip-Wells

275 USD

ELISA Kit

Human growth hormone-binding protein (GHBP) ELISA Kit

growth hormone-binding protein (GHBP)

growth hormone receptor isoform 1; Growth hormone receptor; growth hormone receptor; GH receptor; serum binding protein; somatotropin receptor; growth hormone binding protein; growth hormone receptor; Somatotropin receptorCleaved into the following chain:Growth hormone-binding protein; GH-binding protein; GHBPAlternative name(s):Serum-binding protein

GHBP

GHR; GHR; GHBP; GH receptor; GH-binding protein; GHBP

GHR_HUMAN

Human

638

No cross-reaction with other factors.

Store all reagents at 2-8 degree C.

Samples: Human serum, plasma or cell culture supernatant and organizations in the natural and recombinant GHBP concentration. Assay Type: Sandwich. Detection Range: 200 ng/ml-3.12 ng/ml. Sensitivity: 0.6 ng/ml

Intra-assay Precision: <= 8%. Inter-assay Precision: <= 12%

Principle of the Assay: This experiment use double-sandwich elisa technique and the ELISA Kit provided is typical. The pre-coated antibody is human GHBP monoclonal antibody and the detecting antibody is polyclonal antibody with biotin labeled. Samples and biotin labeling antibody are added into ELISA plate wells and washed out with PBS or TBS. Then Avidin-peroxidase conjugates are added to ELISA wells in order; Use TMB substrate for coloring after reactant thoroughly washed out by PBS or TBS. TMB turns into blue in peroxidase catalytic and finally turns into yellow under the action of acid. The color depth and the testing factors in samples are positively correlated.

4503993

NP_000154.1

NM_000163.4

69,237 Da

Cytokine Signaling In Immune System Pathway (366171); Cytokine-cytokine Receptor Interaction Pathway (83051); Cytokine-cytokine Receptor Interaction Pathway (460); Endochondral Ossification Pathway (198812); Growth Hormone Receptor Signaling Pathway (477128); Immune System Pathway (106386); Jak-STAT Signaling Pathway (83077); Jak-STAT Signaling Pathway (488); Neuroactive Ligand-receptor Interaction Pathway (83053); Neuroactive Ligand-receptor Interaction Pathway (462)

This gene encodes a member of the type I cytokine receptor family, which is a transmembrane receptor for growth hormone. Binding of growth hormone to the receptor leads to receptor dimerization and the activation of an intra- and intercellular signal transduction pathway leading to growth. Mutations in this gene have been associated with Laron syndrome, also known as the growth hormone insensitivity syndrome (GHIS), a disorder characterized by short stature. In humans and rabbits, but not rodents, growth hormone binding protein (GHBP) is generated by proteolytic cleavage of the extracellular ligand-binding domain from the mature growth hormone receptor protein. Multiple alternatively spliced transcript variants have been found for this gene.[provided by RefSeq, Jun 2011]

GH receptor: Receptor for pituitary gland growth hormone involved in regulating postnatal body growth. On ligand binding, couples to the JAK2/STAT5 pathway. Defects in GHR are a cause of Laron syndrome (LARS). A severe form of growth hormone insensitivity characterized by growth impairment, short stature, dysfunctional growth hormone receptor, and failure to generate insulin-like growth factor I in response to growth hormone. Defects in GHR may be a cause of idiopathic short stature autosomal (ISSA). Short stature is defined by a subnormal rate of growth. Belongs to the type I cytokine receptor family. Type 1 subfamily. 4 isoforms of the human protein are produced by alternative splicing. Protein type: Receptor, cytokine; Membrane protein, integral. Chromosomal Location of Human Ortholog: 5p13-p12. Cellular Component: extracellular space; cell surface; integral to plasma membrane; plasma membrane; extracellular region; integral to membrane; receptor complex. Molecular Function: hematopoietin/interferon-class (D200-domain) cytokine receptor activity; protein binding; protein homodimerization activity; growth factor binding; peptide hormone binding; protein kinase binding. Biological Process: succinate metabolic process; oxaloacetate metabolic process; activation of MAPK activity; positive regulation of multicellular organism growth; fatty acid metabolic process; tyrosine phosphorylation of JAK2 protein; response to estradiol stimulus; positive regulation of tyrosine phosphorylation of Stat3 protein; 2-oxoglutarate metabolic process; allantoin metabolic process; receptor internalization; creatinine metabolic process; isoleucine metabolic process; cytokine and chemokine mediated signaling pathway; citrate metabolic process; valine metabolic process; regulation of multicellular organism growth; endocytosis; JAK-STAT cascade; creatine metabolic process; multicellular organismal metabolic process; cellular response to hormone stimulus; positive regulation of peptidyl-tyrosine phosphorylation; positive regulation of tyrosine phosphorylation of Stat5 protein; insulin-like growth factor receptor signaling pathway; response to cycloheximide; taurine metabolic process. Disease: Hypercholesterolemia, Familial; Laron Syndrome

48-Strip-Wells

275 USD

96-Strip-Wells

420